期刊：
INTERNATIONAL JOURNAL OF OPHTHALMOLOGY,2010年10(02):203-208 ISSN：1672-5123
通讯作者：
Peng, QH
作者机构：
[彭清华; 姚小磊; 张菁蓉; 吴权龙] Hunan Univ Tradit Chinese Med, Key Discipline Ophthalmol, Affiliated Hosp 1, Key Discipline Tradit Chinese Ophthalmol, Changsha 410007, Hunan, Peoples R China.;[谭涵宇] Hunan Univ Tradit Chinese Med, Key Discipline Ophthalmol & Otolaryngol Chinese M, Changsha 410007, Hunan, Peoples R China.
通讯机构：
[Peng, QH] Hunan Univ Tradit Chinese Med, Key Discipline Ophthalmol, Affiliated Hosp 1, Key Discipline Tradit Chinese Ophthalmol, Changsha 410007, Hunan, Peoples R China.
关键词：
castrate;dry eye;lacrimal gland;andro-gen receptors;androgen-like activity;extract of Buddleja officinalis;eye drops
摘要：
AIM: To evaluate the effects of the extract of Buddleja officinalis eye drops in basic tears secretory volume, tear film stability, expression of androgen receptors (AR) in castrated rats with dry eye, and to investigate the therapeutic effects of the extract of Buddleja officinalis on dry eye caused by gonadal hormones level imbalance. METHODS: Forty-five Wistar masculinity rats were divided at random into nine groups, including normal groups (A1, A2 and A3); model groups (B1, B2 and B3); therapy groups with extract of Buddleja officinalis eye drops (Cl, (2 and C3). The "1" stood for being fed for 1 month, and "2" for 2 months, and "3" for 3 months. The dry eye model was established with orchiectomy on groups B and C. Group C was treated with Buddleja officinalis extract eye drops for one month. All rats were checked with Schirmer I test (SIT) and tear film break-up time (BUT). Expression of AR was analyzed by flow cytometer (FCM). RESULTS: The SIT value of group C was significantly higher than that of group B (P < 0.01) and the BUT value of group C was significantly longer than that of group B (P < 0.01), which indicated the eye drop could significantly keep basic tears secretory volume and tear film stability. And the expression of AR of group C was much higher than that of group B, which showed that available composition of the eye drops maybe display androgen-like activity. CONCLUSION: The main components of the extract of Buddleja officinalis is the flavonoids that can significantly inhibit happening of dry eye of rat after androgen level lowered. Its mechanism is like androgen's and it can display androgen-like activity to keep basic tears secretory volume and tear film stability
作者机构：
[Xu, Jian] Hunan Univ Tradit Chinese Med, Postgrad Sch, Changsha 410208, Hunan, Peoples R China.;[Peng, Qinghua] Hunan Univ Tradit Chinese Med, Affiliated Hosp 1, Dept Ophthalmol, Changsha 410007, Hunan, Peoples R China.
通讯机构：
[Peng, QH] Hunan Univ Tradit Chinese Med, Affiliated Hosp 1, Dept Ophthalmol, Changsha 410007, Hunan, Peoples R China.
摘要：
Current management of retinitis pigmentosa (RP) includes an attempt at slowing down the degenerative process through therapies that use either Western or traditional Chinese medicine (TCM). Novel therapies in Western medicine (WM) include use of tailor-made gene therapy, transplantation of stem cells, or neuroprotection treatment. TCM treatment includes two major approaches. These are orally applied herbal decoctions and acupuncture. In fact, all TCM treatments are based on the differentiation of a symptom-complex, which is the characteristic essence of TCM. Thus, diagnosed RP may be treated via the liver, the kidney, and the spleen. The principle behind these treatments is to invigorate the blood and brighten the eyes by toning up the liver and the kidney. Also treatments to cope with deficiencies in the two concepts that are unique and fundamental to TCM are considered: Qi or "vital energy" and Yin and Yang or the harmony of all the opposite elements and forces that make up existence. In particular, the Qi deficiency that results from blood stasis is addressed in these treatments. This paper also puts forward the existing problems and the prospect of the future development on integrating TCM with WM.
通讯机构：
[Peng, QH] Hunan Univ Tradit Chinese Med, Affiliated Hosp 1, Dept Ophthalmol, Changsha 410007, Hunan, Peoples R China.
关键词：
dry eye;lacrimal gland;extract of buddleja officinalis;androgen
摘要：
AIM: To assess the effects of extract of Buddleja officinalis on tear secretion volume, tear film stability, expressions of TGF-beta 1, IL-1 beta, TNF-alpha in lacrimal gland of castrated rabbits with dry eye. METHODS: A total of 30 victory rabbits were divided averagely into normal group (A), model group (B), therapy group with low dose extract of Buddleja officinalis (C), therapy group with high dose extract of Buddleja officinalis (D) and therapy group with genistein (E). The dry eye model was established with orchiectomy on Group B, C, D, E. Group C, D, E were administered intragastrically with corresponding dose extract of Buddleja officinalis or genistein for 30 days. All rabbits were detected with SIT. TGF-beta 1, IL-1 beta, TNF-alpha were detected with immunohistochemistry and the ultrastructure of lacrimal gland was observed under transmission electron microscope. RESULTS: The SIT value of group C, D, E were respectively 13.167 +/- 4.957, 14.667 +/- 5.279, 8.667 +/- 0.516, obviously higher than that of group B 5.667 +/- 2.338 (P<0.01). The positive expression of IL-1 beta in acinar cell and glandular tube cell of group C, D were 0.470 +/- 0.048, 0.510 +/- 0.088, obviously lower than that of group B 0.770 +/- 0.118 (P <0.01). The positive expression of TNF-alpha of group C, D were 0.498 +/- 0.156, 0.435 +/- 0.069, obviously lower than that of group B 0.769 +/- 0.095 too (P <0.01). The positive expression of TGF-beta 1 of group C, D were 0.406 +/- 0.171, 0.497 +/- 0.147, obviously higher than that of group B 0.222 +/- 0.113(P<0.01). Any result of group C, D was positive compared with that of group E (P <0.05). Ultrastructure of the lacrimal gland of group C, D, E was well preserved, especially in D group it was remarkable. CONCLUSION: The extract of Buddleja officinalis can adjust lacrimal gland partial inflammation of dry eye.
作者：
Li, Wen-Juan;Peng, Qing-Hua（彭清华）;Tan, Han-Yu;Liu, Yan
期刊：
INTERNATIONAL JOURNAL OF OPHTHALMOLOGY,2012年5(6):667-669 ISSN：2222-3959
通讯作者：
Peng, QH
作者机构：
[Peng, Qing-Hua; Tan, Han-Yu; Li, Wen-Juan; Liu, Yan] Hunan Univ Tradit Chinese Med, Affiliated Hosp 1, Key Disciplines Chinese Med Ophthalmol, Changsha 410007, Hunan, Peoples R China.
通讯机构：
[Peng, QH] Hunan Univ Tradit Chinese Med, Affiliated Hosp 1, Key Disciplines Chinese Med Ophthalmol, Changsha 410007, Hunan, Peoples R China.
关键词：
Qingguangan;trabeculectomy;MMP-2;MMP-9
摘要：
AIM: To explore the effect of Qingguangan on the expressions of MMP-2 and MMP-9 in filtering bleb scarring area after trabeculectomy in rabbit model. METHODS: Thirty-two New Zealand rabbits were randomized into four groups: control group, experimental group, MMC group (ocular trabeculectomy in combination with MMC), and Qingguangan group. Trabeculectomy was performed on both eyes in each group except control group. Qingguangan group was mouth-fed with Qingguangan (solution). On postoperative day 14, the appearances of MMP-2 and MMP-9 on filtrating blebs were observed by immunohistochemistry. RESULTS: Statistical differences of the expressions of MMP-2 and MMP-9 were noted among groups on day 14 following surgery. Histology immunohistochemistry showed significant differences on the expressions of MMP-2 and MMP-9 between each groups( P<0.05). CONCLUSION: Qingguangan can promote the expressions of MMP-2 and MMP-9.
作者机构：
[彭清华] Key Department of Ophthalmology, the First Affiliated Hospital, Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan Province, China. pqhz_520@126.com
通讯机构：
[Peng, Qing-hua] Key Department of Ophthalmology, the First Affiliated Hospital, Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan Province, China.
摘要：
AIM: To explore the effect of Buddleia flavonoids drug-containing plasma and androgen receptor (AR) blocker on the expression of STAT1 phosphoprotein. METHODS: In vitro lacrimal gland epithelial cells were cultivated with H(2)O(2) to establish the dry eye apoptosis state. Blank plasma group, Buddleia officinalis plasma total flavonoids interfere with drug-containing group, and the intervention group of testosterone propionate were set. The expressions of STAT1 phosphoprotein of each group were observed by Western blot. AR blocker flutamide was used to explore the intended androgen effect of Buddleia flavonoids. RESULTS: After the intervention of drug-containing plasma, the expression of STAT1 phosphoprotein in Buddleja officinalis drug-containing plasma intervention group. (0.353 +/- 0.494) and testosterone propionate intervention group (0.502 +/- 0.036) were enhanced and the differences between the two groups were significant (P < 0.01). After using AR blocker, the expression of STAT1 phosphoprotein in each group (0.268 +/- 0.061, 0.283 +/- 0.106, 0.213 +/- 0.071) had no difference. CONCLUSION: Buddleja officinalis drug-containing plasma total flavonoids can promote the expression of STAT1 phosphorylation.
期刊：
Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine,2010年8(03):244-249 ISSN：1672-1977
通讯作者：
Peng, Qing-hua
作者机构：
[彭清华] Key Department of Chinese Traditional Ophthalmology, the First Affiliated Hospital, Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan Province, China. yxlshh@126.com
通讯机构：
[Peng, Qing-hua] Key Department of Chinese Traditional Ophthalmology, the First Affiliated Hospital, Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan Province, China.
摘要：
OBJECTIVE: To explore the possible mechanism of eye drops of Buddleja officinalis extract in treating dry eye of castrated rats by analyzing the expressions of Bax and Bcl-2 proteins. METHODS: Forty-five Wistar male rats were randomly divided into sham-operated group, untreated group and eye drops of Buddleja officinalis Maxim. extract (treatment) group. The dry eye model was established with orchiectomy in the untreated group and treatment group. Rats in the treatment group were treated with eye drops of Buddleja officinalis Maxim. extract, one drop once, three times daily. Eyes of rats in the sham-operated group and untreated group were instilled with normal saline. After one-, two-, or three-month treatment, five rats in each group were scarified respectively. Then samples were taken to detect related indices. Expressions of Bax and Bcl-2 of lacrimal gland were checked by immunohistochemical method and quantity of apoptotic cells was counted. RESULTS: After one-, two- or three-month treatment, the quantities of expressions of Bax in acinar epithelial cells and glandular tube cells were significantly lower, and those of Bcl-2 were significantly higher in the treatment group than in the untreated group, and the quantities of apoptotic cells of the treatment group were significantly lower than those of the untreated group (P<0.01). CONCLUSION: The main components of extract of Buddleja officinalis Maxim. are flavonoids, which can significantly inhibit cell apoptosis in lacrimal gland.
摘要：
Glaucoma, the second leading cause of blindness, is an irreversible optic neuropathy. The mechanism of optic nerve injury caused by glaucoma is undefined at present. There is no effective treatment method for the injury. Stem cells have the capacity of self-renewal and differentiation. These two features have made them become the research focus on improving the injury at present. This paper reviews the application progress on different types of stem cells therapy for optic nerve injury caused by glaucoma.
