期刊论文

Effects of Ganlu Xiaodu Decoction on the Expression of MiR-155 in EV71 Infected Cells

中文

中国中医急症

1004-745X

2016

25

10

1840-1843,1858

10.3969/j.issn.1004-745X.2016.10.003

国家自然科学基金项目（81173188） 湖南中医药大学“中医经典理论与经方应用研究”科技创新团队项目

本校为第一机构

目的 观察甘露消毒丹及其残方对肠道病毒71型(EV71)感染后的RD细胞miRNA-155表达的影响.方法 运用细胞培养技术,设甘全方组、清残方组、利残方组、利巴韦林组、正常细胞对照组、模型对照组,将药物作用于EV71感染后的RD细胞,24 h后RT-PCR法检测各组细胞miR-155表达.结果 1)模型对照组、甘全方组、清残方组、利巴韦林组miR-155表达均比正常细胞对照组低,利残方组比正常细胞对照组升高(P＜ 0.05或P＜ 0.01);甘全方组、清残方组、利残方组miR-155的表达比模型对照组高(P＜ 0.05或P＜0.01),利巴韦林组与模型对照组差异不明显(P＞0.05);甘全方组、清残方组、利残方组miR-155表达比利巴韦林组升高(P＜ ...

Objective： To observe Ganlu Xiaodu decoction and its incomplete prescriptions＇ effects on MiR- 155＇s expression in RD cells infected by EV71. Methods： With technique of cell cuhuring,Ganlu Xiaodu decoction therapy group ,incomplete Qing prescription therapy group ,incomplete Li prescription therapy group ,normal cells control group ,model control group and ribavirin control group were set,and then the expressions of miR-155 in these groups after intervention with drugs in 24 hours were detected by RT-PCR. Results： 1 ）Compared with normal ...