期刊论文

中文

中国中医药信息杂志

1005-5304

2018

25

5

82-85

10.3969/j.issn.1005-5304.2018.05.018

ZYBZH-Y-HUN-24:国家中药标准化项目（中药标准行动计划项目） 2017-23:大学生研究性学习和创新性实验计划

本校为第一机构

目的建立药食两用植物百合中2种有效成分对香豆酸、没食子酸的HPLC含量测定方法。方法采用Hypersil BDC-C18色谱柱(200 mm×4.6 mm,5 µm),对香豆酸的测定以甲醇-0.1%甲酸水溶液(30∶70)为流动相等度洗脱,没食子酸的测定以甲醇-0.1%磷酸水溶液(15∶85)为流动相等度洗脱,检测波长分别为310、270 nm,流速1.0 mL/min,柱温25 ℃,进样量10 µL。结果在上述色谱条件下,对香豆酸、没食子酸与相邻色谱峰之间分离度良好。对香豆酸线性回归方程为Y=1×10~8X+57 335(r=0.998 7),线性范围为10.00~160.03 ng;没食子酸线性回归方程为Y=3×10~7X-21 414(r=0.998 2),线性范围为9.99~159.95 ng。对香豆酸、没食子...

Objective To establish an HPLC method for contents determination of p-coumaric acid and gallic acid in Lilii Bulbus used both as medicine and food. Methods The chromatographic separation was performed on a Hypersil BDC-C18 column (200 mm × 4.6 mm, 5 µm); the column temperature was maintained at 25 ℃. For the p-coumaric acid, isocratic elut of methanol - 0.1% formic acid aqueous solution (30:70) was adopted at the flow rate of 1.0 mL/min. For the gallic acid, isocratic elut of methanol - 0.1% phosphoric acid aqueous solution (15:85) was adopt...