期刊论文

中文

中华中医药杂志

1673-1727

2016

31

8

3241-3246

国家自然科学基金项目（No.81274008）~~；

本校为第一机构

目的:比较补阳还五汤含药血清和含药血浆对缺氧培养的大鼠脑微血管内皮细胞(BMECs)纤溶系统和黏附分子的表达与体内实验真实值的接近程度,为半体内实验选择含药血浆还是含药血清提供参考依据。方法:用颈内动脉线栓法建立局灶性脑缺血模型,缺氧培养箱孵育BMECs,采用ELISA法测定PAI-1、tPA的表达,RT-PCR法检测ICAM-1、VCAM-1 mRNA的含量,Western Blot法测定ICAM-1、VCAM-1蛋白的含量。结果:补阳还五汤含药血浆较含药血清更显著降低缺氧损伤大鼠BMECs PAI-1、增强tPA水平,含药血浆和含药血清均能明显降低ICAM-1、VCAM-1、ICAM-1 mRNA及VCAM-1 mRNA含量,与空白血浆、空白血清比较,差异显著(P

Objective: To compare closeness degree of the expression of fibrinolytic system and adhesion molecules between plasma containing Buyang Huanwu Decoction (BHD) and serum containing on brain microvascular endothelial cells (BMECs) injury by hypoxia in rats with true value of animal in vivo experiments, as well as to provide a reference for the selection of plasma containing or serum containing in the study of ex vivo experiments. Methods: The experiment model of regional cerebral ischemia was established by internal carotid artery line plug method, and BMECs were cultured in hypoxia incubator. T...