作者机构：
[葛金文; 王国佐; 易亚乔; 成绍武] Hunan University of Chinese Medicine, Changsha 410208, China;[方锐] School of Medicine, Hangzhou Normal University, Hangzhou 310018, China;[刘林] First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha 410007, China
通讯机构：
Hunan University of Chinese Medicine, Changsha, China
作者机构：
[李晟; 刘林] School of Nursing, China;[王国佐; 刘洋; 成邵武; 葛金文; 龚胜强; 黄娟; 程成] Key Lab of Hunan Prov. for Prev. and Treatm. of Intgd. Traditional Chinese and W. Med. on Cardiocerebral Dis., Hunan University of Chinese Medicine, Changsha, 410208, China;[秦莉花] School of Nursing, China, Key Lab of Hunan Prov. for Prev. and Treatm. of Intgd. Traditional Chinese and W. Med. on Cardiocerebral Dis., Hunan University of Chinese Medicine, Changsha, 410208, China
期刊：
MOLECULAR MEDICINE REPORTS,2015年11(6):4047-4052 ISSN：1791-2997
通讯作者：
Ge, JW
作者机构：
[Shi, Yong-Mei; Liao, Jun] Hunan Univ Chinese Med, Dept Anat, Changsha 410208, Hunan, Peoples R China.;[Xia, Xing] Hunan Univ Chinese Med, Campus Network Ctr, Changsha 410208, Hunan, Peoples R China.;[Ge, Jin-Wen; Wang, Guo-Zuo] Hunan Univ Chinese Med, Coll Integrated Tradit Chinese & Western Med, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Ge, JW ] H;Hunan Univ Chinese Med, Coll Integrated Tradit Chinese & Western Med, 300 Xueshi St, Changsha 410208, Hunan, Peoples R China.
关键词：
cholesterol;creatinine;cystatin C;genomic DNA;high density lipoprotein;low density lipoprotein;triacylglycerol;uric acid;cation transport protein;herbaceous agent;messenger RNA;metal transporting protein 1;naotaifang;adult;aged;aging;Article;cellular parameters;cholesterol blood level;correlational study;creatinine blood level;creatinine clearance;disease marker;DNA isolation;female;glomerulus filtration rate;groups by age;human;kidney function;leukocyte function;male;middle aged;normal human;protein blood level;restriction fragment;Southern blotting;telomere;telomere length;triacylglycerol blood level;urea nitrogen blood level;uric acid blood level;animal;drug effects;genetics;hippocampus;Infarction, Middle Cerebral Artery;metabolism;pathology;Sprague Dawley rat;upregulation;Rattus;Animals;Cation Transport Proteins;Drugs, Chinese Herbal;Hippocampus;Infarction, Middle Cerebral Artery;Male;Rats, Sprague-Dawley;RNA, Messenger;Up-Regulation
摘要：
The expression of Ferroportin (Fpn) was examined at different time points in rats following focal cerebral ischemia treated with or without the traditional Chinese medicine Naotaifang. Initially, rats were randomly divided into 2, 6, 12, 24 and 72 h groups following middle cerebral artery occlusion (MCAO) and the mRNA and protein level of Fpn was detected by immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR) at the above time points. Secondly, the rats were randomly divided into five groups as follows: Sham surgery group, model group, low-dose group (3 g/kg NTE), medium dose group (9 g/kg NTE) and the high-dose group (27 g/kg NTE). After 3 days of corresponding therapy by intragastric administration once a day, the regional cerebral ischemia model was reproduced by the MCAO suture method. On the third day, the neurological behavior of the rats was analyzed by neurobehavioral assessment. Fpn in the hippocampal CA2 region was measured by immunohistochemistry and the mRNA level of Fpn was detected by RT-PCR. Expression of Fpn in the hippocampal CA2 region reached a peak 12 h after surgery (P<0.05, compared with the model group). The high-dose group (27 g/kg NTE) exhibited a lower neurological behavior score (P<0.05) and a higher level of expression of Fpn at the mRNA and protein level compared with the sham surgery group and model group (P<0.05). Dysregulation of intracellular iron balance is possibly a new mechanism underlying cerebral ischemia. NTE can protect the neuronal population in the hippocampal CA2 region by adjusting the expression of Fpn to balance iron levels following cerebral ischemia.
