期刊论文

中文

中国老年学杂志

1005-9202

2015

35

2

415-418

10.3969/j.issn.1005-9202.2015.02.061

国家自然科学基金（81102593,81273753）； 中国博士后科学基金（20110491258）； 湖南省科技计划项目（2014FJ3023）； 地方高校国家级大学生创新创业训练计划项目（201210541003）； 湖南省中西医结合基础重点学科资助；

本校为第一机构

目的:探讨内质网应激(ERS)在高脂饮食2型糖尿病转基因MKR小鼠脂肪肝发生中的作用。方法高脂饲料诱发MKR鼠形成脂肪肝,观察肝脏形态变化,检测肝脏甘油三酯和脂肪酸水平,RT-PCR检测小鼠肝脏内质网应激相关基因mRNA表达变化。结果与正常小鼠比较, MKR组小鼠肝脏GRP78、XBP1、FAS、ACC-α、GSK3βmRNA表达水平升高(P

Objective To observe the effect of endoplasmic reticulum stress on the formation of liver steatosis in MKR mice raised by high fat feeds. Methods MKR mice were raised by high fat feeds to form liver steatosis. The pathological changes of liver were examined,and the levels of TG and FFA in the liver were examined too. RT-PCR was used to analyze the related gene expressions of endoplasmic reticulum stress in the liver. Results The gene expressions of GRP78,XBP1,FAS,ACC-α and GSK3β on mRNA level in MKR mice were elevated slightly in contrast wit...