期刊论文

中文

中国老年学杂志

1005-9202

2014

34

20

5784-5786

10.3969/j.issn.1005-9202.2014.20.076

湖南省自然科学基金(13JJ3100,09JJ3073) 高等学校博士学科点专项科研基金资助课题(20094323110002)

本校为第一机构

目的：从VEGF-Notch1信号通路观察脑泰方对局灶性脑缺血大鼠的治疗性血管新生样作用。方法将65只SD大鼠随机分为假手术组、MCAO组（模型组）、脑泰方组（NTF）、NTF＋ED（重组人血管内皮抑制素）组。线栓法制备大鼠大脑中动脉栓塞（MCAO）模型，NTF 组及NTF＋ED组分别于造模成功后24 h 灌胃NTF或灌胃NTF加腹腔注射ED，术后1、7、14、28 d 取脑，采用RT-PCR、Western 印迹方法观察血管内皮样生长因子（ VEGF）、Flk-1、Notch1的表达。结果与MCAO组比较，NTF组大鼠各时间点缺血侧脑组织VEGF、Flk-1、Notch1表达水平升高，在第14天时达高峰，而加用血管内皮生成抑制素恩度后，这种增高效应随即消失...

Objective To investigate the therapeutic effects on angiogenesis of Naotangfang extracts by VEGF-Notch1 signaling pathway in ischemic brain damage in rats.Methods 65 SD rats were randomly divided into sham, cerebral ischemia model (MCAO), Naotaifang extract ( NTF) and NTF+Endostatin ( ED) groups.Cerebral ischemia model was established by modified Zea Longa's method.At 24 h post-MCAO, endostatin was injected into abdominal cavity and NTF extract was intragastrically administrated.Rats were sacrificed on days 1, 7, 14 and 28 d post-MCAO.VEGF, Flk-1 and Notch1 protein and mRNA levels in ischemic ...