摘要:
The expression of Ferroportin (Fpn) was examined at different time points in rats following focal cerebral ischemia treated with or without the traditional Chinese medicine Naotaifang. Initially, rats were randomly divided into 2, 6, 12, 24 and 72 h groups following middle cerebral artery occlusion (MCAO) and the mRNA and protein level of Fpn was detected by immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR) at the above time points. Secondly, the rats were randomly divided into five groups as follows: Sham surgery group, model group, low-dose group (3 g/kg NTE), medium dose group (9 g/kg NTE) and the high-dose group (27 g/kg NTE). After 3 days of corresponding therapy by intragastric administration once a day, the regional cerebral ischemia model was reproduced by the MCAO suture method. On the third day, the neurological behavior of the rats was analyzed by neurobehavioral assessment. Fpn in the hippocampal CA2 region was measured by immunohistochemistry and the mRNA level of Fpn was detected by RT-PCR. Expression of Fpn in the hippocampal CA2 region reached a peak 12 h after surgery (P<0.05, compared with the model group). The high-dose group (27 g/kg NTE) exhibited a lower neurological behavior score (P<0.05) and a higher level of expression of Fpn at the mRNA and protein level compared with the sham surgery group and model group (P<0.05). Dysregulation of intracellular iron balance is possibly a new mechanism underlying cerebral ischemia. NTE can protect the neuronal population in the hippocampal CA2 region by adjusting the expression of Fpn to balance iron levels following cerebral ischemia.
摘要:
目的:从VEGF-Notch1信号通路观察脑泰方对局灶性脑缺血大鼠的治疗性血管新生样作用。方法将65只SD大鼠随机分为假手术组、MCAO组(模型组)、脑泰方组(NTF)、NTF+ED(重组人血管内皮抑制素)组。线栓法制备大鼠大脑中动脉栓塞(MCAO)模型,NTF 组及NTF+ED组分别于造模成功后24 h 灌胃NTF或灌胃NTF加腹腔注射ED,术后1、7、14、28 d 取脑,采用RT-PCR、Western 印迹方法观察血管内皮样生长因子( VEGF)、Flk-1、Notch1的表达。结果与MCAO组比较,NTF组大鼠各时间点缺血侧脑组织VEGF、Flk-1、Notch1表达水平升高,在第14天时达高峰,而加用血管内皮生成抑制素恩度后,这种增高效应随即消失。结论脑泰方可能通过上调VEGF-Notch1信号通路的表达以达到治疗性血管新生样作用。
作者:
Jun Liao(廖君);Xing Xia;Guo-zuo Wang;Yong-mei(石咏梅);Shi Jin-wen Ge
作者机构:
[Yong-mei; Jun Liao; Xing Xia; Guo-zuo Wang; Jin-wen Ge] Department of Anatomy,Hunan University of Chinese Medicine;[Yong-mei; Jun Liao; Xing Xia; Guo-zuo Wang; Jin-wen Ge] Campus Network Center,Hunan University of Chinese Medicine;[Yong-mei; Jun Liao; Xing Xia; Guo-zuo Wang; Jin-wen Ge] College of Integrated Medicine,Hunan University of Chinese Medicine;[Yong-mei; Jun Liao; Xing Xia; Guo-zuo Wang; Jin-wen Ge] Hunan Province Key Laboratory of Integrated Medicine for Cardi-Cerebrovascular Disease,Hunan University of Chinese Medicine
摘要:
Objective: To observe the expression ofFerroportin (Fpn) at different time points in rats after focal cerebral ischemia treated with or without Chinese medicine NTE (extract of HuangQi, Chuan Xiong, Di Long, Jiang Can)_Methods: The experiment is carried out by two steps: Firstly , SD rats were randomly divided into 2 h, 6 h, 12 h, 24 h, 72 h groups after operation of Middle cerebral artery occlusion (MCAO), mRNA and protein level of Fpn were detected by immunohistochemistry and RT-PCR at above time points; Secondly, the rats were randomly allotted five groups as following: sham operation group, model group, low dose group of NTE (3 g/kg), medial dose group of NTE (9_g/kg), large dose group of NTE (27 g/kg).After three days of corresponding therapy by intragastric administration once a day, the regional cerebral ischemia model was reproduced by middle cerebral artery occlusion (MCAO)with suture method.Three days later, the rats treated by previous method.The third day, Neurological behavior of rats were detected by neurobehavioral testing.Fpn in Hippocampal C2 region was measured by immunohistochemical method and mRNA of Fpn was detected by RT-PCR.Results: Expression of Fpn in hippocamp C2 reaches the highest point at 12 h after operation (P<0.05); compared with model group, the large dose group of NTE (27 g/kg) displays a lower Neurological behavior score (P<0.05); and large dose group ofNTE showes higher lever expression of Fpn both at mRNA and protein level compared with sham operation group and model group (P<0.05).Conclusion: Disregulation of intracellular iron balance perhaps is the new mechanism of cerebral ischemia injury, the NTE can protect the neuron in Hippocampal C2 area by adjusting the expression of Fpn to balance the iron after cerebral ischemia.