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Development of an Ultra-High Performance Liquid Chromatography Method for Simultaneous Determination of Six Active Compounds in Fructus aurantii and Rat Plasma and Its Application to a Comparative Pharmacokinetic Study in Rats Administered with Different Doses

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成果类型:
期刊论文
作者:
Wang, Wenbo;Zhao, Linlin;Huang, Huiyong(黄惠勇);Yao, Jiamei;Zhou, Lu;...
通讯作者:
Qiu, XJ
作者机构:
[Wang, Wenbo; Zhou, Lu; Wang, Dongsheng; Qiu, Xinjian; Qiu, XJ] Cent South Univ, Inst Integrated Tradit Chinese & Western Med, Xiangya Hosp, Changsha, Hunan, Peoples R China.
[Zhao, Linlin] Cent South Univ, Xiangya Hosp 3, Phys Examinat Ctr, Changsha, Hunan, Peoples R China.
[Huang, Huiyong; Qiu, Xinjian] Hunan Univ Tradit Chinese Med, Prov Key Lab TCM Diagnost, Changsha, Hunan, Peoples R China.
[Yao, Jiamei] Cent South Univ, Xiangya Hosp, Dept Gerontol, Changsha, Hunan, Peoples R China.
通讯机构:
[Qiu, XJ ] C
Cent South Univ, Inst Integrated Tradit Chinese & Western Med, Xiangya Hosp, Changsha, Hunan, Peoples R China.
语种:
英文
关键词:
acetic acid;acetonitrile;aurantiin;caffeine;diphenhydramine;fructus aurantii decoction;herbaceous agent;hesperetin;hesperidin;luteolin;meranzin hydrate;naringenin;neohesperidin;unclassified drug;animal experiment;animal model;area under the curve;Article;chemical analysis;chemical composition;clinical assessment;concentration response;controlled study;drug blood level;drug determination;drug megadose;drug stability;flow rate;Fructus aurantii;fruit;low drug dose;male;nonhuman;pharmacokinetic parameters;quantitative analysis;rat;ultra performance liquid chromatography
期刊:
Journal of Analytical Methods in Chemistry
ISSN:
2090-8865
年:
2018
卷:
2018
期:
1
页码:
7579136
基金类别:
0is work was supported by the China Postdoctoral Science Foundation (no. 2015M572254), National Natural Science Foundation of China (nos. 81202807 and 81703963), Sui-Yu Hu Inheritance Studio of National Prominent Chinese Medicine Doctor, and State Administration of Traditional Chinese Medicine (no. 2014.20).
机构署名:
本校为其他机构
院系归属:
中医学院
中医诊断研究所
摘要:
A rapid, accurate, and sensitive ultra-high performance liquid chromatography (UHPLC) method was established for simultaneously detecting naringin, hesperidin, neohesperidin, meranzin hydrate, naringenin, and hesperetin in Fructus aurantii (FA) decoction. Analysis was performed on Waters BEH (R) C18 (50 mm × 2.1 mm, 1.7 μm) at a flow rate of 0.2 mL/min by using (A) acetonitrile and (B) 0.5% acetic acid-water as the mobile phase. The method was well validated on linearity, precision, recoveries, and stability. Then, we used the same UHPLC cond...

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