期刊论文

Investigation of Experimental Technique of Tannerella forsythia Cultured in Vitro

中文

湖南中医药大学学报

1674-070X

2018

38

1

43-46

10.3969/j.issn.1674-070X.2018.01.011

2016年度湖南省大学生研究性学习和创新性实验计划项目（1021-001017094）。

本校为第一机构

目的 探索福赛斯坦纳菌生长繁殖的基本条件,比较N-乙酰胞壁酸及不同营养因子对其生长繁殖的影响,筛选该菌体外培养的最佳营养条件,建立其体外培养的技术方法.方法 (1)培养基制备:在血平板中加0.005％N-乙酰胞壁酸形成改良培养基;制备血平板与不同浓度(0.5、3、10、50、100 mg/L)的N-乙酰胞壁酸形成的培养基;制备10 mg/L N-乙酰胞壁酸与氯化血红素、酵母提取物、维生素K3、脱纤维羊血组合形成不同组别的培养基.(2)接种培养后,用磷酸盐缓冲液将细菌洗脱,并用酶标仪(OD600)测定其吸光度.结果 福赛斯坦纳菌在含N-乙酰胞壁酸的培养基中生长更好,浓度为10 mg/L时促进作用最明显且细菌形态更为典型.在组...

Objective To investigate the basic requirements for the growth and proliferation of Tannerella forsythia and compare the growth-promoting effects with different nutritional factors and screen the optimum culture in vitro conditions and establish the technique of culturing it. Methods (1)Preparation of culture medium: 0.005％ N-acetylmuramic acid was added to the blood agar medium, forming the modified medium. Different concentration of N-acetylmuramic acid (0.5, 3, 10, 50, 100 mg/L)was added to the blood agar medium. The different mediums of N-...