期刊论文

Intervention of Buddleja officinalis total flavonoids drug-containing plasma on the expression of AR mRNA in dry eye apoptosis model

Peng, Q.-H.

中文

国际眼科杂志

1672-5123

2011

11

2

220-222

10.3969/j.issn.1672-5123.2011.02.07

中国国家自然科学基金资助项目（No.30772824） 中国教育部高等学校博士学科点专项基金资助项目（No.200805410004） 中国湖南省自然科学基金资助项目（No.07JJ3049） 中国湖南省科技厅科研基金资助项目（No.200-9FJ3001） 2008年中国湖南省研究生创新基金资助项目

本校为第一机构

目的:探讨密蒙花总黄酮含药血浆对雄激素水平下降所致干眼症的细胞凋亡模型AR mRNA表达的影响,并应用雄激素受体阻滞剂以确定AR mRNA表达与密蒙花总黄酮含药血浆和AR受体的结合效应有关.方法:体外分离及培养泪腺上皮细胞,以H2O2诱导大鼠泪腺上皮细胞凋亡,建立雄激素水平下降所致干眼症的细胞凋亡模型.设立空白血浆组、密蒙花总黄酮含药血浆组、丙酸睾酮组,分别观察各组AR mRNA表达情况;并应用雄激素受体阻滞剂氟他胺,观察密蒙花总黄酮的拟雄激素效应.结果:密蒙花总黄酮含药血浆干预组与丙酸睾酮干预组中AR mRNA的表达增强,两组间的差异有显著性(P0.05).结论:密蒙花总黄酮含药血浆可促进AR mRNA表...

AIM: To establish the dry eye apoptosis model caused by the reduction of androgen levels, to explore the effect of Buddleja officinalis total flavonoids drug-containing plasma for activating the expression of AR mRNA; detect the expression of AR mRNA with the same method after using androgen receptor blockers, to determine relationship of the expression of AR mRNA with the binding effect of AR receptor in the Buddleja officinalis total flavonoids drug-containing plasma. METHODS: In vitro lacrimal gland epithelial cells were isolated and cultivated. The apoptosis of epithelial cells of rat lacr...