期刊论文

中文

中华中医药杂志

1673-1727

2018

33

4

1344-1347

国家自然科学基金项目（No.81460597）； 贵州省科技基金项目（No.黔科合基础[2017]1009）； 贵州省科技厅与贵阳中医学院联合基金项目（No.黔科合LH字[2016]7109号）； 湖南省高校科技创新团队《感染性疾病中医药防治研究》资助项目（No.15）~~；

本校为第一机构

目的:从体内外2个方面探讨仙茅多糖作为流感疫苗佐剂对巨噬细胞的抗原提呈能力和释放活性因子的影响。方法:在体外实验中,以小鼠巨噬细胞株RAW264.7为研究对象,用流式细胞仪检测不同浓度的仙茅多糖对细胞表面CD80、CD86和MHC-Ⅱ表达量的影响,用ELISA法检测细胞培养上清中TNF-α、IL-1、IL-6的水平;在体内实验中,以昆明种小鼠作为研究对象,仙茅多糖与流感疫苗同时注射对小鼠进行免疫,免疫3d后提取各组小鼠腹腔巨噬细胞,对其免疫效果进行评价。结果:在体外实验中,不同浓度的仙茅多糖刺激RAW264.7细胞后,细胞表面CD80、CD86和MHC-Ⅱ都有不同程度表达上调,同时使培养液中TNF-α、IL-1和IL-6含量上升...

Objective: To discuss the effects of curculigo orchioides polysaccharide (COP) as adjuvant of influenza vaccine on antigen presenting ability and releasing activity factors of macrophage, from the two aspects both in vivo and in vitro. Methods: In vitro experiment, the mouse macrophage cell line RAW264.7 was studied. The effects of different doses curculigo orchioides polysaccharide on the expression levels of CD80, CD86 and MHC-II were detected by FCM. The levels of TNF-α, IL-1 and IL-6 in cell culture supernatants were detected by ELISA. In ...