Identification and validation of microRNAs as endogenous controls for quantitative polymerase chain reaction in plasma for stable coronary artery disease
This work was supported by the National Natural Science Foundation of China (Nos. 81302883) and the Natural Science Foundation of Hunan Province (Nos.14JJ4056). The microarray experiments were performed by Bo Hao Bio-tech, Shanghai, China.
机构署名：
本校为第一且通讯机构
院系归属：
医学院
中医学院
摘要：
Background: Circulating microRNAs (miRNAs) have been proved to serve as biomarkers for diagnosis and assessment of prognosis of coronary artery disease (CAD). Reverse transcription quantitative polymerase chain reaction (RT-qPCR) is a widely-used technique to estimate expression levels of circulating miRNAs. Selection of optimal endogenous control (EC) remains critical to obtain reliable qPCR data of miRNAs expression. However, reference controls for normalization of circulating miRNA in CAD are still lacking. The purpose of this study was to i...
