Identification and validation of microRNAs as endogenous controls for quantitative polymerase chain reaction in plasma for stable coronary artery disease
作者：
Zhang, Yuejuan;Tang, Wenxian;Peng, Ling;Tang, Jinqiang
（唐金强）;Yuan, Zhaokai
（袁肇凯） 期刊：
CARDIOLOGY JOURNAL,2016年23(6):694-703 ISSN：1897-5593
通讯作者：
Yuan, ZK
作者机构：
[Tang, Jinqiang; Zhang, Yuejuan] Hunan Univ Chinese Med, Coll Med, Dept Biochem & Mol Biol, Changsha, Hunan, Peoples R China.;[Tang, Wenxian] Hunan Normal Univ, Coll Life Sci, Changsha, Hunan, Peoples R China.;[Yuan, Zhaokai; Peng, Ling] Hunan Univ Chinese Med, Coll Tradit Chinese Med, Res Inst TCM Diag, Changsha, Hunan, Peoples R China.
通讯机构：
[Yuan, ZK ] H;Hunan Univ Chinese Med, Coll Tradit Chinese Med, Res Inst TCM Diag, Changsha, Hunan, Peoples R China.
关键词：
*circulating microRNA;*normalization;*reference genes;*reverse transcription quantitative polymerase chain reaction (RT-qPCR);*stable coronary artery disease
摘要：
Background: Circulating microRNAs (miRNAs) have been proved to serve as biomarkers for diagnosis and assessment of prognosis of coronary artery disease (CAD). Reverse transcription quantitative polymerase chain reaction (RT-qPCR) is a widely-used technique to estimate expression levels of circulating miRNAs. Selection of optimal endogenous control (EC) remains critical to obtain reliable qPCR data of miRNAs expression. However, reference controls for normalization of circulating miRNA in CAD are still lacking. The purpose of this study was to identify stably expressed miRNAs to normalize RT-qPCR data derived from plasma in stable CAD. Methods: We identified 10 stably expressed candidate ECs by combining miRNA microarray screening and literature screening. These 10 candidate ECs were estimated by RT-qPCR and the data were analyzed by NormFinder and BestKeeper algorithm. Results: Two most stable ECs were identified as EC candidates and they were subsequently validated in another larger cohort. The 2 candidates were also validated by normalizing the expression levels of miR-21. In general, they were superior to the commonly used reference gene RNU6 in quantification cycle (Cq) value, stability value and normalization effect. Conclusions: Our results demonstrated that miR-6090 and miR-4516 can be used as reference genes for plasma miRNA analysis in stable CAD. © 2016 Via Medica.
语种：
英文
展开
促排卵汤对不孕大鼠卵巢局部基质金属蛋白酶mRNA表达的研究
期刊：
湖南中医药大学学报,2004年24(4):18-19+22 ISSN：1674-070X
作者机构：
[唐金强; 刘海燕] 湖南中医学院;怀化市中医院 湖南长沙410007;湖南怀化418000;指导;[袁懿] 湖南省怀化市中医院
关键词：
促排卵汤;不孕症;基质金属蛋白酶;动物模型;大鼠
摘要：
目的观察促排卵汤对雄激素致不孕大鼠(ASR)卵巢局部基质金属蛋白酶的作用.方法采用ASR大鼠模型及原位杂交技术,观察大鼠卵巢局部基质金属蛋白酶MMP-9mRNA表达的变化.结果促排卵汤能促进不孕大鼠卵巢局部基质金属蛋白酶MMP-9 mRNA表达,和模型组相比差异有显著性意义(P＜0.01).结论促排卵汤具有促进不孕大鼠卵巢局部基质金属蛋白酶MMP-9mRNA表达的作用,是其促进卵巢排卵的可能机制.
语种：
中文
展开
桃仁蛋白梯度聚丙烯酰胺凝胶电泳的研究
期刊：
中草药,2003年34(4):378-379 ISSN：0253-2670
作者机构：
湖南中医学院基础部生物化学教研室;湖南中医学院基础部中医诊断研究所;湖南中医学院第一附属医院药剂科药检室;湖南中医学院基础部生物化学教研室 湖南长沙410007;[唐金强] 唐金强
关键词：
桃仁;蛋白梯度;聚丙烯酰胺凝胶电泳;分离蛋白质;药材鉴定;电泳方法;行分离;网状物;载体;物质;立体;聚合;带电
摘要：
电泳作为一种分离蛋白质的手段已是含蛋白中药材鉴定的常用方法.聚丙烯酰胺凝胶电泳(PAGE)是利用丙烯酰胺和甲叉双丙烯酰胺聚合形成的立体网状物作为载体对不同的带电物质进行分离的电泳方法.
语种：
中文
展开
