期刊论文

中文

湖南中医药大学学报

1674-070X

2009

29

3

9-12

10.3969/j.issn.1674-070X.2009.03.004

[基金项目）国家自然科学基金资助项目（30772824） 湖南省自然科学基金资助项目（07JJ3049） 教育部高等学校博士学科点专项基金项目（200805410004）.

本校为第一机构

目的 建立围绝经期性激素水平下降导致的干眼症兔模型,为进一步探讨围绝经期性激素水平下降导致干眼症的病理机制和实验研究创造条件.方法 取12只成年雌兔,分为空白组(A)和模型组(B),模型组采用双侧卵巢切除术,两组均饲养45 d,采用Schirmer Ⅰ试验观察其基础泪液分泌量、测定泪膜破裂时间现察其泪膜稳定性的改变,处死进行形态学(光镜、电镜、免疫组织化学)检测.结果 干眼症免模型建立后,模型组(B)Schirmer Ⅰ试验测量值明显低于空白组(A),泪膜破裂时间明显短于空白组(A),差异均有显著统计学意义(P

Objective To establish a rabbit model wiht dry eye syndrome caused by sex hormones decline in peri-menopause, in order to create the conditions for further study of the pathogenesis and experiment of the dry eye syndrome. Methods 12 adult female rabbits were divided into blank control group （A） and model group （B） in which the double ootheetomy were operated, all the rabbits were fed for 45 days. The basic tear secretion and the tear film break-up time were measured to observe the changes in tear film stability with Schirmer test and then a...