期刊论文

中文

北京中医药大学学报

1006-2157

2017

40

4

290-296

10.3969/j.issn.1006-2157.2017.04.006

国家自然科学基金青年项目（No.81503442、No.81603470） 湖南省教育厅科研项目（No.11C0953、No.16B196）

本校为第一机构

目的观察六味地黄汤对慢性肾衰模型大鼠肾组织中脯氨酸羟化酶2(PHD2)、缺氧诱导因子-1α(HIF-1α)表达的影响,探讨慢性肾衰的发病机制以及六味地黄汤防治慢性肾脏病的分子机制。方法60只SD大鼠随机分为假手术组,模型组,六味地黄汤低剂量、中剂量和高剂量组。5/6肾切除法复制慢性肾衰模型。六味地黄汤低剂量、中剂量和高剂量组分别给予3.38 g/(kg·d)、6.75 g/(kg·d)、13.5 g/(kg·d)六味地黄汤灌胃。假手术组和模型组给予10 mL/(kg·d)生理盐水灌胃,每天1次,连续灌胃12周。造模12周后,检测各组大鼠24 h尿蛋白、血尿素氮和血肌酐,HE染色观察各组大鼠肾组织形态学变化,免疫组化法和RT-PCR法分别检...

Objective To investigate the effects of Liuwei Dihuang Tang (LWDHT,Six-Ingredient Rehmannia Decoction) on the expression of proline Hydroxylase2 (PHD2) and hypoxia inducible factor alpha-1 (HIF-1α) in renal tissue of rats with chronic renal failure,and to investigate the pathogenesis and molecular mechanism of LWDHT in preventing and treating chronic kidney disease. Methods 60 SD rats were randomly divided into sham-operation group,model group,LWDHT low-dose,medium-dose and high-dose groups,and chronic renal failure model group (5 /6 nephrecto...