期刊论文

中文

临床与病理杂志

2095-6959

2011

31

2

93-97

10.3969/j.issn.1673-2588.2011.02.001

湖南省教育厅科研课题（07C479）~~；

本校为第一机构

目的：探讨还少丹对D-半乳糖（D-galactose,D-gal）诱导的衰老模型小鼠心肌线粒体结构与功能的影响。方法：将小鼠随机分为空白对照组、衰老模型组、还少丹低、高剂量组。采用D-gal建立衰老模型,还少丹水煎液灌胃6周。以差速离心法分离小鼠心肌组织线粒体;Comas亮蓝蛋白定量法测定线粒体蛋白含量;分光光度法检测线粒体呼吸链复合体I和复合体Ⅳ的活性,以及Na^＋-K^＋-ATP酶和Ca^2＋-ATP酶的活性。结果：衰老模型组复合体I和复合体Ⅳ的活性明显降低（分别为P〈0.05和P〈0.01）,还少丹低、高剂量组均能明显提高复合体I和复合体Ⅳ的活性（P〈0.01）。衰老模型组Na^＋-K^＋-ATP酶和Ca^2＋-ATP酶活性明...

Objective To investigate the effect of huanshaodan on myocardial mitochondrial structure and function in aged mice induced by D-galactose（D-gal）.Methods Mice were divided randomly into a blank control group,an aged model group,a low dose huanshaodan group,and a high dose huanshaodan group.The aged model was established by injecting D-gal.After feeding with huanshaodan for 6 weeks,mitochondria were obtained from heart of mice by differential centrifugation.Protein content of mitochondria was determined by coomassie blue staining.Then spectroph...