期刊论文

Deng, C.-Q.

中文

结合医学学报：英文版

2095-4964

2009

7

11

1078-1085

10.3736/jcim20091111

国家自然科学基金资助项目（No.30572301）；

本校为第一且通讯机构

目的:研究补阳还五汤及其有效组分和三七总皂苷（total Panax notoginseng saponins,TSPN）含药血浆对血小板衍生生长因子（platelet-derived growth factor,PDGF）诱导的血管平滑肌细胞（vascular smooth musclecell,VSMC）增殖的影响。方法:采用血浆药理学方法及 PDGF—BB 诱导 VSMC 增殖模型。以甲基噻唑基四唑（methyl thiazolyl tet-razolium,MTT）法检测空白血浆对 VSMC 生长的影响和含药血浆对 PDGF—BB 诱导的 VSMC 增殖的半数抑制浓度,确定补阳还五汤及其有效组分和三七总皂苷用药浓度。MTT 法和流式细胞术检测补阳还五汤及有效组分和 TSPN、阿托伐他汀对 VSMC 增殖活性及细胞周期的影...

OBJECTIVE: To explore the effects of plasma containing Buyang Huanwu Decoction (BYHWD), a compound of traditional Chinese medicine, and its effective components (alkaloids and glycosides) and total Panax notoginseng saponins (TSPN) on vascular smooth muscle cell (VSMC) proliferation induced by platelet-derived growth factor (PDGF) in vitro. METHODS: VSMC proliferation was induced by incubating VSMCs with PDGF-BB. Effects of different concentrations of blank plasma on the VSMC proliferation and 50% inhibitory concentrations of plasma containing BYHWD, its alkaloids and glycosides, TSPN and ator...