期刊论文

Xiang, Q

英文

Microchimica Acta

0026-3672

2017

184

6

1847-1854

10.1007/s00604-017-2194-3

We would like to thank National Natural Science Foundation of hunan province (2016JJ3098), Outstanding Youth Scientific Research Project Funded by Education department of Hunan Province (15B169), Science and technology Innovation Team in Colleges and Universities in Hunan Province《Chinese traditional medicine for treatment of infectious diseases》(No: 15, Grxjb-7), Doctor start-up Foundation of Hunan University of Chinese Medicine (9982-1001019), Key Subjects of Hunan University of Chinese Medicine《pathogenic biology》(NO.1) and Project Funded by Hunan Provincial Level Course《Immunology and pathogenic biology》(No. 48) for the financial support.

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The authors describe an aptamer based fluorometric assay for the determination of ATP. It is based on deoxyribonuclease I-aided target recycling and signal amplification. The DNA probe consists of two regions (sequences) that represent the capture probe and the signal probe, respectively. In the absence of ATP, the probe is adsorbed by the surface of graphene oxide (GO) via π-stacking interactions. This results in quenching of the fluorescent label (carboxyfluorescein) and protects it from being cleaved by DNase. Upon adding ATP, the probe wil...