期刊论文

Study on effect target of Maxing Shigan decoction on anti-influenza virus A in vitro

中文

湖南中医药大学学报

1674-070X

2008

28

2

5-9

10.3969/j.issn.1674-070X.2008.02.002

湖南省自然科学基金资助项目（06JJ2055）； 湖南省卫生厅科研课题（2006-56）； 湖南省科技计划项目（2007f4144）；

本校为第一机构

目的 研究麻杏石甘汤体外抗A型流感病毒的作用靶点.方法 运用细胞培养技术、血凝试验和MTT法检测麻杏石甘汤在4种给药方式中对流感病毒血凝滴度和流感病毒感染的MDCK细胞活性的影响.结果 在直接灭活病毒作用中,麻杏石甘汤在3.19～31.25 mg/mL浓度范围内的血凝滴度低于病毒对照组,在15.63～31.25mg/mL时病毒血凝滴度低于利巴韦林组;在干预病毒吸附作用中,麻杏石甘汤在1.95～31.25 mg/mL浓度范围内病毒血凝滴度低于病毒对照组,在7.81～31.25 mg/mL时病毒血凝滴度低于刺巴韦林组;在保护宿主细胞作用中,麻杏石甘汤在3.19～31.25 mg/mL浓度范围内病毒血凝滴度低于病毒对照组,在7.81～31.25 mg/mL时病...

Objective To study the effect target of Maxing Shigan decoction （MXSGD） on anti-influenza Virus A in vitro. Methods The cell culture technique, the Hemagglutination reaction and MTT test were used to determine the effects of MXSGD on hemagglutination titer of influenza virus A and MDCK cells activities infected by influenza virus A with 4 different treatment methods. Results When the concentration of MXSGD for killing virus was from 3.19 mg/mL to 31.25 mg/mL, the hemagglutination titer was lower obviously compared with virus control group; fr...