期刊论文

中文

中药材

1001-4454

2016

39

9

2081-2085

10.13863/j.issn1001-4454.2016.09.038

湖南省教育厅优秀青年项目（15B179） 湖南中医药大学科技创新团队项目（201503） 湖南中医药大学博士科研启动项目（1001-017） 湖南中医药大学中医诊断学国家重点学科开放基金项目（2015ZYZD19,2015ZYZD16）

本校为第一机构

目的:探讨四君子汤促进胃肠黏膜损伤修复的作用及机制。方法:采用血清药理学方法制备四君子汤含药血清;tips划痕法建立IEC-6细胞迁移模型,观察四君子汤含药血清对ICE-6细胞迁移的影响;实时细胞分析仪(RTCA)检测四君子汤含药血清对IEC-6细胞增殖的影响;荧光定量PCR(qRT-PCR)法检测TLR-2和MyD88 mR-NA表达;Western blot法检测TLR-2与MyD88蛋白表达。结果:中(10%)、高(20%)浓度四君子汤含药血清可促进IEC-6细胞迁移(P

Objective: To study the effect and mechanism of Sijunzi decoction( SJZD) containing serum on the repairing of gastrointestinal mucosal damage. Methods: SJZD containing serum was prepared by serum pharmacological method. Cell migration model was established by tips scratch method, Real-time-cell-analyzer( RTCA) was used to mensurate IEC-6 cell proliferation, the mRNA and protein expression of TLR-2 and My D88 mRNA were detected by qRT-PCR and Western blot analysis,respectively. Results: Medium dose( 10%) and high dose( 20%) of SJZD containing serum stimulated IEC-6 cell migration at 8 h after c...