期刊论文

Study on relativity between Ginsenoside Rgl antagonizing cell injury and autophagy of PC12 cells induced by oxygen glucose deprivation/reoxygenation

中文

中华中医药杂志

1673-1727

2017

32

5

1974-1978

湖南省科技厅一般项目(No.2014SK3001) 湖南省研究生科研创新项目(No.CX2016B372)~~

本校为第一机构

目的:探讨人参皂苷Rgl (Rgl)对PC12细胞氧糖剥夺后再复糖复氧(OGD/R)诱导的细胞自噬性损伤的作用及作用机制。方法:以PC12细胞建立OGD/R自噬性损伤模型,观察Rgl抗OGD/R后细胞自噬性损伤的作用,并从PI3KI/Akt/m-TOR和PI3KⅢ/Becline-l/Bcl-2信号通路研究其作用机制。结果:Rgl能增加OGD/R诱导的PC12细胞存活、减少乳酸脱氢酶(LDH)漏出;同时抑制细胞自噬体的形成,降低胞浆LC3-Ⅱ阳性斑片的数量、下调LC3-Ⅱ/LC3-I蛋白比值而上调p62蛋白表达。机制研究结果表明,Rgl提高了PI3KI、Akt、m-TOR蛋白磷酸化水平,对PI3KⅢ、Becline-1和Bcl-2蛋白表达没有影响。结论:在缺糖缺氧2h再复糖复氧24h,细胞出现过度...

Objective: To explore the effect and mechanism of Ginsenoside Rgl (Rgl) antagonizing PC12 cells autophagy injuryinduced by oxygen glucose deprivation/reoxygenation (OGD/R). Methods: Autophagy injury model of PC 12 cells induced by OGD/R was established, the effect of Rgl on autophagy injury was observed, and the mechanism was studied through PI3KI/Akt/m-TOR and PI3KⅢ/Becline-l/Bcl-2 signaling pathways. Results: Rgl could increase cells survive and decreasedLactate dehydrogenase (LDH) leakage induced by OGD/R, reduce autophagosome and LC3 Ⅱ po...