期刊论文

Effects of Maxing Shigan Decoction Decocted by Different Methods and Its Drug Containing Serum on Neuraminidase Activity of Influenza A Virus

中文

中国中西医结合杂志

1003-5370

2016

36

9

1119-1123

10.7661/CJIM.2016.09.1119

国家自然科学基金资助项目（No.81072751＆81473468） 湖南省自然科学基金资助项目（No.13JJ6062） 湖南省教育厅重点资助项目（No.13A066） 湖南省卫生厅科研课题资助项目（No.2010014）

本校为第一机构

目的探讨不同煎煮方法的麻杏石甘汤及其含药血清对A型流感病毒神经氨酸酶(NA)活性的影响。方法以2 - (4-methylumbellifery1) - α-D-N-acetyl neuraminic acid(MUNANA)为底物,检测不同煎煮方法的麻杏石甘汤药液、相应含药血清以及抑制病毒增殖作用中含药血清对流感病毒NA活性的影响。结果(1)药液对流感病毒NA活性的影响:麻黄先煎25 min组和麻黄先煎30 min组的病毒NA荧光值低于4药同煎组(P < 0.05,P < 0.01); (2)含药血清对流感病毒NA活性的影响:麻黄先煎30 min组、麻黄先煎40 min组的NA荧光值低于4药同煎组(P < 0.05,P < 0.01); (3)抑制病毒增殖作用中,含药血清对流感病毒NA活性的影响:麻黄先煎...

Objective To observe the effects of Maxing Shigan Decoction (MSD) decocted by different methods and its drug containing serum on neuraminidase (NA) activity of influenza A virus (IAV). Methods The effects of MSD decocted by different methods,its corresponding drug containing serums,and drug containing serum in inhibiting the proliferation of virus on NA activity of IAV were detected using 2-(4-methyl umbellifery1) -α-D-Nacetyl neuraminic acid (MUNANA) as substrate. Results (1) Effect of MSD on NA activity of IAV: OD value was less in groups wi...