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A Graphene Oxide–Based Sensing Platform for the Determination of Methicillin-Resistant Staphylococcus aureus Based on Strand-Displacement Polymerization Recycling and Synchronous Fluorescent Signal Amplification:

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成果类型:
期刊论文、会议论文
作者:
Ning, Yi(宁毅);Gao, Qiang(高强);Zhang, Xiaoqing;Wei, Ke(魏科);Chen, Lingli*陈伶利
通讯作者:
Chen, Lingli(陈伶利
作者机构:
[Wei, Ke; Ning, Yi; Gao, Qiang; Chen, Lingli] Hunan Univ Chinese Med, Dept Microbiol, Sch Med, Xueshi Rd 300, Changsha 410208, Hunan, Peoples R China.
[Zhang, Xiaoqing] Hunan Univ Chinese Med, Dept Inorgan Phys Chem, Sch Pharm, Changsha, Hunan, Peoples R China.
通讯机构:
[Chen, Lingli] H
Hunan Univ Chinese Med, Dept Microbiol, Sch Med, Xueshi Rd 300, Changsha 410208, Hunan, Peoples R China.
语种:
英文
关键词:
graphene oxide;methicillin-resistant Staphylococcus aureus;mecA gene;signal amplification;biosensor
期刊:
SLAS Discovery
ISSN:
2472-5552
年:
2016
卷:
21
期:
8
页码:
851-857
会议名称:
中华中医药学会免疫学分会第二次学术交流会暨中华中医药学会免疫学分会青年委员会成立大会
会议论文集名称:
中华中医药学会免疫学分会第二次学术交流会暨中华中医药学会免疫学分会青年委员会成立大会论文集
会议时间:
2016-08-27
会议地点:
深圳
会议赞助商:
中华中医药学会
基金类别:
National Natural Science Foundation of Hunan ProvinceNatural Science Foundation of Hunan Province [2016JJ3098]; Outstanding Youth Scientific Research Project - Education Department of Hunan Province [15B169]; National Natural Science FoundationNational Natural Science Foundation of China (NSFC) [81202451]; Science and Technology Innovation Team in Colleges and Universities in Hunan Province (Chinese traditional medicine for treatment of infectious diseases) [15, Grxjb-7]; Doctor Start-Up Foundation of Hunan University of Chinese Medicine [9982-1001019]; Key Subjects of Hunan University of Chinese Medicine; Hunan Provincial Level Course [48]
机构署名:
本校为第一且通讯机构
院系归属:
医学院
药学院
摘要:
To develop new technology for detecting methicillin-resistant Staphylococcus aureus (MRSA), a novel fluorescent biosensor based on Klenow fragment (KF)-assisted target recycling amplification and synchronous fluorescence analysis was created. Carboxy-fluorescein (FAM)-labeled single-stranded DNA (ssDNA) containing a capture probe and a signal probe was adsorbed onto the surface of graphene oxide (GO) via π-stacking interactions, resulting in the fluorescence quenching of the dye. When target and primer were introduced, the fluorescence was res...

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