M13 phage peptide ZL4 exerts its targeted binding effect on schistosoma japonicum via alkaline phosphatase

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成果类型：

期刊论文

作者：

Liu, Yan;Yang, Shenghui（杨胜辉）;Xiao, Jianhua;Yu, Liang;Chen, Li（陈丽）;...

通讯作者：

Zeng, Qingren

作者机构：

[Liu, Yan; Xiao, Jianhua; Yu, Liang; Wang, Kegeng; Zou, Ju] Univ South China, Affiliated Hosp 1, Dept Parasitol, Hengyang, Peoples R China.

[Tan, Sijie] Univ South China, Affiliated Hosp 1, Dept Histol & Embryol, Sch Med, Hengyang, Peoples R China.

[Yu, Zhengyang] Univ South China, Affiliated Hosp 1, Dept Surg Oncol, Hengyang, Peoples R China.

[Yang, Shenghui] Hunan Univ Chinese Med, Sch Med, Dept Prevent Med & Pathogen Biol, Changsha, Hunan, Peoples R China.

[Chen, Li] Univ Penn, Dept Med, Perelman Sch Med, Philadelphia, PA 19104 USA.

通讯机构：

[Zeng, Qingren] C

Cent S Univ, Xiangya Sch Med, Ctr Cell & Mol Biol Expt, 172 Tongzipo Rd, Changsha 410013, Hunan, Peoples R China.

语种：

英文

关键词：

MppZL4;Schitosoma japonicum;alkaline phosphatase

期刊：

International Journal of Clinical and Experimental Pathology

ISSN：

1936-2625

年：

2015

卷：

期：

页码：

1247-1258

基金类别：

Hunan Provincial Young Foundation of China [11B106]; China University Doctor Fund [201036000Z110]; Hunan Provincial Natural Science Foundation of ChinaNatural Science Foundation of Hunan Province [11JJ3106]

机构署名：

本校为其他机构

院系归属：

医学院

摘要：

The present study was to determine the targeting effect of M13 phage peptide ZL4 (MppZL4) on Schistosoma japonicum (S.j). Mice infected with S.j were injected with MppZL4. Real-time PCR was used to detect the distribution and metabolism of MppZL4 in the livers and lungs of mice. In vivo refusion test was performed to detect the targeting of MppZL4. Western blotting was employed to determine the expression of MppZL4. Live imaging was used to detect the distribution of oligopeptide MppZL4. Immunohistochemistry was employed to determine MppZL4 location on adult S.j body surface. Gomori method was...

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M13 phage peptide ZL4 exerts its targeted binding effect on schistosoma japonicum via alkaline phosphatase

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