期刊论文

中文

中国中医急症

1004-745X

2014

23

12

2182-2183,2199

10.3969/j.issn.1004-745X.2014.12.014

12C0278:湖南省教育厅科学研究项目 12C0269:湖南省教育厅科学研究项目

本校为第一机构

目的 探讨益肾活血汤对大鼠肾间质纤维化的拮抗作用及机制.方法 将60只大鼠随机分为假手术组、模型组、益肾活血汤治疗组、贝那普利治疗组.制备单侧输尿管梗阻(UUO)大鼠模型,在术后第21日处死大鼠留取梗阻侧肾组织观察肾间质病理变化,并用免疫组化法测定肾组织中纤溶酶原激活剂抑制物-1(PAI-1)、转化生长因子-β(TGF-β)及结缔组织生长因子(CTGF)的表达,处死前留取24 h尿测定尿蛋白,心腔穿刺取血测定血清尿素氮(BUN)、血清肌酐(Scr).结果 益肾活血汤组24h尿蛋白、BUN、Scr水平、肾间质纤维化程度及PAI-1、TGF-β、CTGF的表达量均明显低于模型组(P＜0.05),但与贝那普利组差异无统计学意义(P＞0.0...

Objective：To investigate the antagonizing effect and mechanism of Yishenhuoxue decoction on renal interstitial fibrosis of unilateral ureteral obstruction （UUO） rats.Methods：Sixty SD rats were randomly assigned to shame operation group,operation model group,Yishenhuoxue decoction group and Benazepril group after unilateral ureteral obstruction.Rats were killed on the 21 day after operation.Renal tissues were examined by HE stain.Immunohistochemistry was performed for plasminogen activator inhibitor-1 （PAI-1）,transforming growth factor-β ...