期刊论文

中文

解剖学杂志

1001-1633

2008

31

4

537-540

10.3969/j.issn.1001-1633.2008.04.026

湖南省自然科学基金（JJY3051）

本校为其他机构

目的：探讨脑源性神经营养因子（BDNF）对出生后大鼠周围神经髓鞘化的作用及其作用机制。方法：用光镜和电镜方法检测坐骨神经纤维的髓鞘化情况；用免疫印迹方法检测坐骨神经p75的表达；用免疫荧光方法检测坐骨神经雪旺细胞内核转录因子（NF-KB）的表达。结果：出生后3d，与对照组相比，实验组坐骨神经髓鞘化的神经纤维数目减少，p75表达下调，雪旺细胞内NF-KB转移率下调；出生后14d，与对照组相比，实验组坐骨神经出现较多的髓鞘化异常。结论：内源性BDNF影响出生后大鼠周围神经的髓鞘化，尤其在早期作用明显；内源性BDNF主要通过p75和NF-KB信号途径调节出生后大鼠周围神经的髓鞘化。

Objective： To investigate the effect of endogenous brain-derived neurotrophic factor （BDNF） on the myelination of the postnatal peripheral nerve in rats. Methods.. The myelination of the sciatic nerve was measured with light microscope and electron microscope; the expression of p75 in the sciatic nerve was measured by Western blot; the nuclear transiocation of NF-kappa B in the sciatic nerve were showed by immunofluorescence. Results ： At postnatal day 3 （P3）, the number of my- elinating fibres, the p75 expression and the rate of NF-kappa...