期刊：
MOLECULAR MEDICINE REPORTS,2015年11(6):4047-4052 ISSN：1791-2997
通讯作者：
Ge, JW
作者机构：
[Shi, Yong-Mei; Liao, Jun] Hunan Univ Chinese Med, Dept Anat, Changsha 410208, Hunan, Peoples R China.;[Xia, Xing] Hunan Univ Chinese Med, Campus Network Ctr, Changsha 410208, Hunan, Peoples R China.;[Ge, Jin-Wen; Wang, Guo-Zuo] Hunan Univ Chinese Med, Coll Integrated Tradit Chinese & Western Med, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Ge, JW ] H;Hunan Univ Chinese Med, Coll Integrated Tradit Chinese & Western Med, 300 Xueshi St, Changsha 410208, Hunan, Peoples R China.
关键词：
cholesterol;creatinine;cystatin C;genomic DNA;high density lipoprotein;low density lipoprotein;triacylglycerol;uric acid;cation transport protein;herbaceous agent;messenger RNA;metal transporting protein 1;naotaifang;adult;aged;aging;Article;cellular parameters;cholesterol blood level;correlational study;creatinine blood level;creatinine clearance;disease marker;DNA isolation;female;glomerulus filtration rate;groups by age;human;kidney function;leukocyte function;male;middle aged;normal human;protein blood level;restriction fragment;Southern blotting;telomere;telomere length;triacylglycerol blood level;urea nitrogen blood level;uric acid blood level;animal;drug effects;genetics;hippocampus;Infarction, Middle Cerebral Artery;metabolism;pathology;Sprague Dawley rat;upregulation;Rattus;Animals;Cation Transport Proteins;Drugs, Chinese Herbal;Hippocampus;Infarction, Middle Cerebral Artery;Male;Rats, Sprague-Dawley;RNA, Messenger;Up-Regulation
摘要：
The expression of Ferroportin (Fpn) was examined at different time points in rats following focal cerebral ischemia treated with or without the traditional Chinese medicine Naotaifang. Initially, rats were randomly divided into 2, 6, 12, 24 and 72 h groups following middle cerebral artery occlusion (MCAO) and the mRNA and protein level of Fpn was detected by immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR) at the above time points. Secondly, the rats were randomly divided into five groups as follows: Sham surgery group, model group, low-dose group (3 g/kg NTE), medium dose group (9 g/kg NTE) and the high-dose group (27 g/kg NTE). After 3 days of corresponding therapy by intragastric administration once a day, the regional cerebral ischemia model was reproduced by the MCAO suture method. On the third day, the neurological behavior of the rats was analyzed by neurobehavioral assessment. Fpn in the hippocampal CA2 region was measured by immunohistochemistry and the mRNA level of Fpn was detected by RT-PCR. Expression of Fpn in the hippocampal CA2 region reached a peak 12 h after surgery (P<0.05, compared with the model group). The high-dose group (27 g/kg NTE) exhibited a lower neurological behavior score (P<0.05) and a higher level of expression of Fpn at the mRNA and protein level compared with the sham surgery group and model group (P<0.05). Dysregulation of intracellular iron balance is possibly a new mechanism underlying cerebral ischemia. NTE can protect the neuronal population in the hippocampal CA2 region by adjusting the expression of Fpn to balance iron levels following cerebral ischemia.
作者：
Jun Liao（廖君）;Xing Xia;Guo-zuo Wang;Yong-mei Shi（石咏梅）;Jin-wen Ge
作者机构：
[Yong-mei Shi; Jun Liao; Xing Xia; Guo-zuo Wang; Jin-wen Ge] Department of Anatomy,Hunan University of Chinese Medicine;[Yong-mei Shi; Jun Liao; Xing Xia; Guo-zuo Wang; Jin-wen Ge] Campus Network Center,Hunan University of Chinese Medicine;[Yong-mei Shi; Jun Liao; Xing Xia; Guo-zuo Wang; Jin-wen Ge] College of Integrated Medicine,Hunan University of Chinese Medicine;[Yong-mei Shi; Jun Liao; Xing Xia; Guo-zuo Wang; Jin-wen Ge] Hunan Province Key Laboratory of Integrated Medicine for Cardi-Cerebrovascular Disease,Hunan University of Chinese Medicine
摘要：
Objective: To observe the expression ofFerroportin (Fpn) at different time points in rats after focal cerebral ischemia treated with or without Chinese medicine NTE (extract of HuangQi, Chuan Xiong, Di Long, Jiang Can)_Methods: The experiment is carried out by two steps: Firstly , SD rats were randomly divided into 2 h, 6 h, 12 h, 24 h, 72 h groups after operation of Middle cerebral artery occlusion (MCAO), mRNA and protein level of Fpn were detected by immunohistochemistry and RT-PCR at above time points; Secondly, the rats were randomly allotted five groups as following: sham operation group, model group, low dose group of NTE (3 g/kg), medial dose group of NTE (9_g/kg), large dose group of NTE (27 g/kg).After three days of corresponding therapy by intragastric administration once a day, the regional cerebral ischemia model was reproduced by middle cerebral artery occlusion (MCAO)with suture method.Three days later, the rats treated by previous method.The third day, Neurological behavior of rats were detected by neurobehavioral testing.Fpn in Hippocampal C2 region was measured by immunohistochemical method and mRNA of Fpn was detected by RT-PCR.Results: Expression of Fpn in hippocamp C2 reaches the highest point at 12 h after operation (P<0.05); compared with model group, the large dose group of NTE (27 g/kg) displays a lower Neurological behavior score (P<0.05); and large dose group ofNTE showes higher lever expression of Fpn both at mRNA and protein level compared with sham operation group and model group (P<0.05).Conclusion: Disregulation of intracellular iron balance perhaps is the new mechanism of cerebral ischemia injury, the NTE can protect the neuron in Hippocampal C2 area by adjusting the expression of Fpn to balance the iron after cerebral ischemia.
作者：
AN CHEN（陈安）;Hui WANG;Xiao-Qion WU;Yong-mei SHI（石咏梅）;Xue-Gang LUO;...
作者机构：
Department of Anatomy, Traditional Chinese Medicine University of Hunan, Changsha 410007, China;Department of Neurobiology, Central South University, Changsha 410013, China;Institute of Neurosciences, The Fourth Military Medical University, Xi'an 710032, China
会议名称：
中国神经科学学会第四次会员代表大会暨第七届全国学术会议(The 7th Biennial Meeting and the 5th Congress of the Chinese Society for Neuroscience)
会议时间：
2007-10-24
会议地点：
杭州
会议主办单位：
中国神经科学学会
会议论文集名称：
中国神经科学学会第四次会员代表大会暨第七届全国学术会议(The 7th Biennial Meeting and the 5th Congress of the Chinese Society for Neuroscience)论文集
摘要：
Objective To observe the effect ofBuyang Huanwu Decoction (BYHWD) on neuron protection.Methods SD rats were given BYHWD or distilled water via gastrogavage after right rubrospinal tract(RST) transection at the level between C3 and C4,8 week after treatment,rubrospinal neurons(RNs) were counted and mean cell size was examined by Nissl staining; concentration of cAMP in red nucleus was determined by radioimmunoassay; changes of BDNF and TrkB expression in RN was examed using immunohistochemistry.Results The number and mean cell size of RN was decreased by 22% and 35% respectively in the BYHWD group,42% and 64% respectively in the distilled water group; the expressions of cAMP,BDNF and TrkB were significantly increased in BYHWD group compared with distilled water group.Conclusion The present results suggest that administration of BYHWD following RST transection leads to promotion axotomized neuron survival and prevention their atrophy,the protective effects on axotomized neuron may be related to its modulation of cAMP and the increase of BDNF and TrkB on RN induced by cAMP.
