期刊论文

Establishment of dry eye model on perimenopause female rabbits with low sexual hormone

中文

眼科新进展

1003-5141

2009

29

1

5-11

10.13389/j.cnki.rao.2009.01.004

30772824:国家自然科学基金 07JJ3049:湖南省自然科学基金

本校为第一机构

目的 建立围绝经期性激素水平下降所致的干眼症白兔模型,为探讨围绝经期性激素水平下降导致干眼症的发病机制和实验治疗提供依据.方法 对6只成年白兔采用双侧卵巢切除术制作干眼症模型,术后饲养45 d,同时取6只正常白兔作空白对照,2组均采用Schirmer Ⅰ试验观察其基础泪液分泌量、测定泪膜破裂时间观察其泪膜稳定性的改变,并进行形态学(光镜、免疫组织化学)检测.结果 白兔模型建立后,模型兔Schirmer Ⅰ实验测量值明显低于正常白兔(P＜0.01)、泪膜破裂时间明显短于正常白兔(P＜0.01),泪腺导管及腺泡上皮细胞中IL-1β、TNF-α、Fas、FasL、Bax阳性表达的细胞数均明显高于正常白兔(P＜0.01),而TGF-1...

Objective To establish the experimental dry eye model caused by low sexual hormone on the perimenopause rabbits, to offer helps for investigating etiopathogenesis and experimental therapy. Methods Six female rabbit models were established with ovariectomy and then fed for 45 days. Six normal rabbits were treated as control group. Rabbits of both two groups were detected SIT, breakup time of tear film （BUT） with Schirmer I experiment to observe the changes of tear film stability. Morphological changes were also observed with light microscope a...