期刊论文

DNA polymerase delta-interacting protein 1 enhances GFP protein degradation

Zhang, B.

中文

细胞与分子免疫学杂志

1007-8738

2014

30

4

363-365

10.13423/j.cnki.cjcmi.008362

国家自然科学基金（81072751） 湖南省高校科技创新团队资助项目（编号15） 湖南省重点学科《中西医综合基础》建设项目（100602）

本校为第一机构

目的证明过表达DNA聚合酶δ相互作用蛋白1（PDIP1）能否促进外源性GFP蛋白的降解。方法将HEK293细胞共转染表达质粒pEGFP-C3与pCMV-myc-PDIP1,利用荧光显微镜检测细胞绿色荧光强度,然后利用Western blot法检测GFP与PDIP1蛋白表达量的关系,再利用氯化铵与MG132分别进行干预,并检测GFP与PDIP1蛋白表达量的关系。结果共转染表达质粒pEGFP-C3与pCMV-myc-PDIP1的HEK293细胞绿色荧光强度明显低于单转pEGFP-C3质粒的细胞;随着PDIP1蛋白表达量的增大,GFP蛋白的表达量减少;利用MG132干预可以明显抑制PDIP1对GFP的降解,而氯化铵干预没有显示明显的效应。结论过表达PDIP1可以促进外源性GFP蛋白的降解,且这一...

OBJECTIVE: To prove whether the over-expression of DNA polymerase delta-interacting protein 1 (PDIP1) protein can promote the degradation of exogenous GFP protein. METHODS: The plasmids of pEGFP-C3 and pCMV-myc-PDIP1 were co-transfected into HEK293 cells. The fluorescence intensity of the transfected cells was analyzed using fluorescence microscope. The expressions of GFP and PDIP1 were measured by Western blotting. Then, the expressions of GFP and PDIP1 were again detected in the cells which were treated by ammonium chloride or MG132, respectively. RESULTS: Compared with the ones transfected ...