期刊论文

中文

辽宁中医药大学学报

1673-842X

2014

16

11

68-70

10.13194/j.issn.1673-842x.2014.11.023

湖南省中医药管理局资助项目（60010288） 湖南省教育厅资助项目（14C0870）

本校为第一机构

目的:观测水通道蛋白在慢性应激肝郁脾虚证模型大鼠胃肠黏膜中的表达.方法:30只大鼠随机分为正常组、模型组及逍遥丸组,采用慢性应激加过度疲劳及饮食失节法复制大鼠肝郁脾虚证模型.采用免疫组化法检测各组大鼠胃肠黏膜组织中AQP3、AQP8的表达.结果:①胃肠黏膜AQP3:与正常组比较,模型组大鼠胃、小肠、结肠黏膜中AQP3表达均显著降低(P＜0.01);与模型组比较,逍遥丸组胃、小肠、结肠黏膜中AQP3表达均上调(P＜0.05,P＜0.01).②胃肠黏膜AQP8:与正常组比较,模型组大鼠胃、小肠、结肠黏膜中AQP8表达均显著降低(P＜0.01);与模型组比较,逍遥丸组胃、小肠、结肠黏膜中AQP8表达上调(P＜0.05,P＜0.01).结论:...

Objective：To observe the expressions of aquaporins in gastrointestinal mucosa of chronic stress liver-stagnation and spleen-deficiency syndrome model rats.Methods：Thirty rats were randomly divided into normal group,model group and Xiaoyao pills group.The rats model of liver-stagnation and spleen-deficiency syndrome was established by chronic stress,fatigue and improper diet.The method of immunocytochemistry was used to determine the expression of aquaporin-3（AQP3）and aquaporin-8（AQP8）in gastrointestinal mucosa of rats.Results：1The gastro...