会议论文

中文

2012

397-403

2012年朱文峰学术思想研讨会暨中医诊断师资班30周年纪念大会

2012年朱文峰学术思想研讨会暨中医诊断师资班30周年纪念大会论文集

2012-10-01

长沙

中华中医药学会

国家自然科学基金资助项目(NO.81072779)

本校为第一机构

目的:探讨通过补肾解毒和健脾解毒两种不同药物血浆体外作用树突状细胞后改善慢乙肝患者DCs的功能,比较哪种方法更能促进DCs激活T细胞的功能提高。方法:前后抽取HBV慢性感染免疫耐受期和免疫清除期共20例患者外周血60ml,分离培养出成熟的树突状细胞,在培养第7天加入补肾解毒与健脾解毒复方中药药物血浆对DCs进行体外干预,促进其成熟,另设空白血浆组对照,培养至第9天,负载HBVcore18-27核心

　　Objective To explore Function improvement of chronic hepatitis b patients by two different drug plasma o the kidney and spleen detoxification acting on the dendritic cells, comparisoning which method is more can promote DCs activate T cell function to improve.Methods Extraction peripheral blood 60 ml of 20 patients of chronic HBV infection immune-tolerant phase and immune clearance period, separation develop mature dendritic cells, external intervention to DCs with the kidney and spleen to detoxify and detoxification compound Chinese medici...