优质葛资源精深加工关键技术及应用
项目作者：
刘向前
项目作者单位：
湖南中医药大学
资助经费：
15万
立项时间：
2013
项目级别：
厅局市级
项目来源：
长沙市科技计划项目
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天茄子镇痛抗炎作用的物质基础研究
项目作者：
盛文兵
项目作者单位：
湖南中医药大学
项目批准号：
13C676
资助经费：
0.8万
立项时间：
2013
项目级别：
厅局市级
项目来源：
省教育厅科研项目
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植物水培体系生物转化生产活性成分的研究
项目作者：
彭菲
项目作者单位：
湖南中医药大学
项目批准号：
2013SK3081
资助经费：
3万
立项时间：
2013
项目级别：
省部级
项目来源：
湖南省科技计划项目
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炮制火候与姜不同炮制品化学成分映对关系的研究
项目作者：
石继连
项目作者单位：
湖南中医药大学
项目批准号：
12JJ3091
资助经费：
3万
立项时间：
2013
项目级别：
省部级
项目来源：
湖南省自然科学基金
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海洋生物来源中药材质量标准研究
项目作者：
王炜
项目作者单位：
湖南中医药大学
资助经费：
33万
立项时间：
2013
项目级别：
国家级
项目来源：
国家重点基础研究发展计划（863计划）
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湘西北土家族药物提取物和标准品库的构建
项目作者：
王炜
项目作者单位：
湖南中医药大学
项目批准号：
201215
资助经费：
3万
立项时间：
2013
项目级别：
厅局市级
项目来源：
湖南省中医药科研计划项目
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Simultaneous Quantification of 18 Bioactive Constituents in Tripterygium wilfordii Using Liquid Chromatography-Electrospray Ionization-Mass Spectrometry
作者：
Zeng, Feng;Wang, Wei;Guan, Shuhong;Cheng, Chunru;Yang, Min;...
期刊：
Planta Medica ,2013年79(9):797-805 ISSN：0032-0943
通讯作者：
Guo, De-an
作者机构：
[Guo, De-an; Zeng, Feng] Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China.;[Guan, Shuhong; Guo, De-an; Yang, Min; Cheng, Chunru; Zeng, Feng] Chinese Acad Sci, Shanghai Res Ctr Modernizat Tradit Chinese Med, Natl Engn Lab TCM Standardizat Technol, Shanghai Inst Biol Sci,Shanghai Inst Mat Med, Shanghai, Peoples R China.;[Wang, Wei] Hunan Univ Chinese Med, Sch Pharm, TCM, Changsha 410208, Hunan, Peoples R China.;[Wang, Wei] Hunan Univ Chinese Med, Sch Pharm, Ethnomed Innovat & Dev Lab, Changsha 410208, Hunan, Peoples R China.;[Avula, Bharathi; Khan, Ikhlas A.] Univ Mississippi, Pharmaceut Sci Res Inst, Natl Ctr Nat Prod Res, Sino US TCM Res Ctr, University, MS 38677 USA.
通讯机构：
[Guo, De-an] P;Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, 38 Xueyuan Rd, Beijing 100191, Peoples R China.
关键词：
Tripterygium wilfordii;sesquiterpenoid alkaloids;diterpenoids;triterpenoids;HPLC;ESI;MS;multiple reaction monitoring;Celastraceae
摘要：
A liquid chromatography–electrospray ionization–mass spectrometry method with
multiple reaction monitoring was established for simultaneous quantification
of 18 bioactive constituents from the stem and root of Tripterygium
wilfordii Hook. f. collected from different places in China and
various commercial preparations. The chromatographic separations were
achieved on an Agilent Poroshell SB-C18 column (150 × 4.6 mm,
3.5 µm) with gradient elution using acetonitrile and 0.03 % formic acid
aqueous solution in 45 min. Detection was performed in the positive
ionization mode by monitoring the precursor–product combination. The
validation of the method included tests of linearity, sensitivity,
precision, repeatability, stability, and accuracy. All calibration curves
showed good linearity (r > 0.9990) within the test range. The
established method showed good precision and accuracy with intraday and
interday variations of 2–5 % and 1–4 %, respectively, and recoveries of
95.5–104.5 %.
语种：
英文
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Anthraquinone derivatives from Rumex plants and endophytic Aspergillus fumigatus and their effects on diabetic nephropathy
作者：
Yang, Yang;Yan, Yong-Ming;Wei, Wei;Luo, Jie;Zhang, Lan-Sheng;...
期刊：
Bioorganic & Medicinal Chemistry Letters ,2013年23(13):3905-3909 ISSN：0960-894X
通讯作者：
Cheng, Yong-Xian
作者机构：
[Zhang, Lan-Sheng; Luo, Jie; Yan, Yong-Ming; Wang, Peng-Cheng; Cheng, Yong-Xian; Yang, Yang] Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650204, Peoples R China.;[Yang, Yong-Xun] Chengdu Univ Tradit Chinese Med, Coll Pharm, Chengdu 610075, Peoples R China.;[Yang, Yang] Univ Chinese Acad Sci, Beijing 100039, Peoples R China.;[Wei, Wei; Zhou, Xiao-Jiang] Hunan Univ Chinese Med, Coll Pharm, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Cheng, Yong-Xian] C;Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650204, Peoples R China.
关键词：
Anthraquinone;Aspergillus fumigatus;C-glycoside;Diabetic nephropathy;Endophytic;Oxanthrone;Rumex
摘要：
Two new oxanthrone C-glycosides, patientosides A (14) and B (15), together with three known ones (11-13), were isolated from Rumex patientia. Their structures were identified on the basis of spectroscopic methods. The absolute configuration for 14 and 15 were deduced by analysis of their CD spectra and comparison with those of known similar compounds. Compounds 11-15, and 14 known anthraquinones (1-4, 6-10, 16-20) previously isolated from Rumex nepalensis, Rumex hastatus, and endophytic Aspergillus fumigatus, respectively, as well as a commercially available compound rhein (5) were evaluated for their inhibitory effects on IL-6 and extracellular matrix production in mesangial cells. © 2013 Elsevier Ltd. All rights reserved.
语种：
英文
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Phenolics from Ageratina adenophora Roots and Their Phytotoxic Effects on Arabidopsis thaliana Seed Germination and Seedling Growth
作者：
Zhou, Zhong-Yu;Liu, Wan-Xue;Pei, Gang;Ren, Hui;Wang, Jing;...
期刊：
Journal of Agricultural and Food Chemistry ,2013年61(48):11792-11799 ISSN：0021-8561
通讯作者：
Tan, Jian-Wen
作者机构：
[Zhou, Zhong-Yu; Xu, Qiao-Lin; Xie, Hai-Hui; Ren, Hui; Tan, Jian-Wen; Wang, Jing] Chinese Acad Sci, South China Bot Garden, Key Lab Plant Resources Conservat & Sustainable U, Guangzhou 510650, Guangdong, Peoples R China.;[Liu, Wan-Xue; Wan, Fang-Hao] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China.;[Pei, Gang] Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.;[Xu, Qiao-Lin; Ren, Hui; Wang, Jing] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China.
通讯机构：
[Tan, Jian-Wen] C;Chinese Acad Sci, South China Bot Garden, Key Lab Plant Resources Conservat & Sustainable U, Guangzhou 510650, Guangdong, Peoples R China.
关键词：
Ageratina adenophora;phenolic compounds;phytotoxic;allelochemical;Arabidopsis
摘要：
A bioassay-directed phytochemical study was conducted to investigate potential allelochemicals in the roots of the invasive plant Ageratina adenophora. Eleven phenolic compounds, including seven new ones, 7-hydroxy-8,9-dehydrothymol 9-O-trans-ferulate (1), 7-hydroxythymol 9-O-trans-ferulate (2), 7,8-dihydroxythymol 9-O-trans-ferulate (3), 7,8-dihydroxythymol 9-O-cis-ferulate (4), methyl (7R)-3-deoxy-4,5-epoxy-d-manno- 2-octulosonate 8-O-trans-p-coumarate (5), methyl (7R)-3-deoxy-4,5-epoxy-d-manno- 2-octulosonate 8-O-cis-p-coumarate (6), and 3-(2-hydroxyphenyl)propyl methyl malonate (7), were isolated from a bioactive subfraction of the ethanol extract of the roots of A. adenophora. The new structures were established on the basis of detailed spectroscopic analysis. The potential phytotoxic effects of these compounds on the germination of Arabidopsis thaliana seeds were tested by a filter paper assay. Compound 7 and known compounds 3-(2-hydroxyphenyl)-1- propanol (8) and o-coumaric acid (9) remarkably showed inhibition activity against Arabidopsis seed germination at a concentration of 1.0 mM. Compounds 1, 2, 5, 6, and 10 showed slight inhibitory activity at the test concentration after treatment for 3 days, while the other compounds showed no obvious inhibitory effects. Moreover, 7-9 were further found to show obvious inhibitory activity on retarding the seedling growth of Ar. thaliana cultured in soil medium. ©2013 American Chemical Society.
语种：
英文
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CURCUMIN TRINICOTINATE REGULATE THE PROLIFERATION OF VSMCS VIA CAVEOLIN-1/ERK1/2 SIGNALING PATHWAY
作者：
Yang, W.;Li, T. P.;Tuo, Q. H.;Zheng, X.;Guo, Z. F.;...
期刊：
Cardiology ,2013年125(SUPPL-2):465-465 ISSN：0008-6312
作者机构：
[Yang, W.; Zheng, X.; Liao, D. F.; Li, T. P.] Univ South China, Dept Pharm & Pharmacol, Hengyang, Hunan, Peoples R China.;[Guo, Z. F.; Tuo, Q. H.] Hunan Univ Chinese Med, Sch Pharm, Changsha, Hunan, Peoples R China.
语种：
英文
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新型京尼平衍生物的合成、表征及抗糖尿病活性
作者：
Li Zhi-Wei* ;Bao Zhi-Chao;Wang Jian-Nan;Cao Rui-Feng;Xie Zhao-Yang;...
期刊：
高等学校化学学报 ,2013年34(12):2752-2758 ISSN：0251-0790
通讯作者：
Li Zhi-Wei
作者机构：
[Wang Jian-Nan; Cao Rui-Feng; Xie Zhao-Yang; Bao Zhi-Chao; Zheng Qun-Yi; Li Zhi-Wei] Natsource Chem Changsha Corp, Changsha 410329, Hunan, Peoples R China.;[Li Zhi-Wei] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410114, Hunan, Peoples R China.;[Zheng Qun-Yi] Hunan Acad Chinese Med, Sch Pharmaceut Sci, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Li Zhi-Wei] N;Natsource Chem Changsha Corp, Changsha 410329, Hunan, Peoples R China.
关键词：
糖尿病;京尼平;抑制剂
摘要：
为了寻找新型的抗糖尿病分子, 从京尼平出发, 经过甲基化(苄基化)、 甲磺酰基取代、 叠氮基取代和还原等4步反应得到含有活泼胺基的2个母核化合物. 这2个母核化合物与不同的酰氯反应, 共得到13个新的京尼平衍生物, 总收率41%~63%, 其结构经核磁共振、 质谱和元素分析确认. 活性测试结果表明, 所有目标化合物在10 mmol/L 浓度下都有一定的抗糖尿病活性, 其中化合物10f对二肽基肽酶4(DPP Ⅳ)的抑制率达31.2%.
语种：
中文
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Molecular Acidity of Singly and Doubly Substituted Phenols: Predictions from Density Functional Reactivity Theory and Hammett Constants
作者：
Liu Liang-Hong;Zhang Peng-Fei* ;Huang Ying
期刊：
物理化学学报 ,2013年29(3):508-515 ISSN：1000-6818
通讯作者：
Zhang Peng-Fei
作者机构：
[Liu Liang-Hong; Huang Ying] Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.;[Zhang Peng-Fei] Cent S Univ, Xiangya Hosp, Minist Hlth, Key Lab Canc Prote, Changsha 410008, Hunan, Peoples R China.
通讯机构：
[Zhang Peng-Fei] C;Cent S Univ, Xiangya Hosp, Minist Hlth, Key Lab Canc Prote, Changsha 410008, Hunan, Peoples R China.
关键词：
Molecular acidity;Density functional reactivity theory;Hammett constant;Molecular electrostatic potential;Natural atomic orbital;Phenol
摘要：
Accurate prediction of molecular acidity with ab initio and density functional theory approaches is of great interest, but remains a challenging task. Density functional reactivity theory (DFRT) with quantum descriptors, such as molecular electrostatic potential and valence natural atomic orbital energies, has recently been developed and used for this purpose. Our previous study of substituted benzoic acids revealed a novel approach to quantitatively predict molecular acidity using the Hammett constant, which gave the same prediction accuracy as that of DFRT. In this work, we applied these two approaches to singly and doubly substituted phenol systems, a total of 83 molecules, confirming their effectiveness and robustness. High accuracy was obtained using both approaches, with the DFRT approach achieving slightly higher accuracy than the Hammett approach in general. These results shed further light on the molecular features governing physiochemical properties such as acidity and basicity, both locally on the atomic level and globally on the functional group or molecular level. Our present results confirm the validity of the sum rule of Hammett constants for doubly and multiply substituted compounds.
语种：
英文
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Preparation of D-tartaric Acid Modified Multi-walled Carbon Nanotubes for Resolution of Propranolol Enantiomers
作者：
Peng, Dong-Ming;Yu, Lin-Yan;Yu, Jin-Gang* ;Jiao, Fei-Peng;Peng, Zhi-Guang;...
期刊：
Current Nanoscience ,2013年9(5):631-634 ISSN：1573-4137
通讯作者：
Yu, Jin-Gang
作者机构：
[Zhang, Ting; Jiao, Fei-Peng; Peng, Dong-Ming; Yu, Jin-Gang; Peng, Zhi-Guang; Yu, Lin-Yan] Cent S Univ, Minist Educ, Key Lab Resources Chem Nonferrous Met, Changsha 410083, Hunan, Peoples R China.;[Peng, Dong-Ming] Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.;[Zhang, Ting; Jiao, Fei-Peng; Yu, Jin-Gang; Peng, Zhi-Guang; Yu, Lin-Yan] Cent S Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.
通讯机构：
[Yu, Jin-Gang] C;Cent S Univ, Minist Educ, Key Lab Resources Chem Nonferrous Met, Changsha 410083, Hunan, Peoples R China.
关键词：
Multi-walled carbon nanotubes;D-tartaric acid;functionalization;enantioseparation;thin-layer chromatography;propranolol.
摘要：
In this paper, multi-walled carbon nanotubes (MWCNTs) were oxidized by 30 w% hydrogen peroxide to introduce hydroxyl groups and carboxyl groups on it. Catalyzed by p-toluenesulfonic acid, D-tartaric acid (DTA) was grafted onto MWCNTs by an esterification reaction. Fourier transform infrared spectroscopy and high resolution transmission electron microscopy were used to elucidate the introduced groups and the morphologies changes of the MWCNTs samples. The D-tartaric acid modified MWCNTs were used as chiral im pregnating reagents for thin-layer chromatography for enantioseparation of propranolol enantiomers, and the chiral separation factor was achieved over 7.23 by a mixed solvent of acetonitrile-tert-butanol-acetic acid (volume ratio is 49:49:2) as developing solvent.
语种：
英文
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Variations in the Levels of Mulberroside A, Oxyresveratrol, and Resveratrol in Mulberries in Different Seasons and during Growth
作者：
Zhou, Jin;Li, Shun-xiang* ;Wang, Wei;Guo, Xiao-yi;Lu, Xiang-yang;...
期刊：
The Scientific World Journal ,2013年2013:380692 ISSN：2356-6140
通讯作者：
Li, Shun-xiang
作者机构：
[Wang, Wei; Li, Shun-xiang; Zhou, Jin; Huang, Dan; Cao, Liang; Guo, Xiao-yi] Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.;[Wei, Bao-yang; Li, Shun-xiang; Zhou, Jin; Lu, Xiang-yang] Hunan Agr Univ, Sch Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Yan, Xin-pei] Hunan Inst Sericulture, Changsha 410127, Hunan, Peoples R China.
通讯机构：
[Li, Shun-xiang] H;Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.
摘要：
This study aimed to investigate the composition of three major stilbenes (mulberroside A, oxyresveratrol, and resveratrol) in different portions of mulberries collected in different seasons and their change molds during growth by high-performance liquid chromatography. Mulberroside A levels were the highest in the bark and roots of Morus atropurpurea Roxb, Morus alba Linn, and Morus latifolia Poir. Oxyresveratrol levels were the highest in roots and stem. Both of these high levels were in September. The amount of resveratrol was very low in all samples. In the stem, Morus latifolia Poir contained more mulberroside A than the other two mulberries. Mulberroside A was not detected in the leaves of the three mulberries. In Morus atropurpurea Roxb seedlings, the root tended to contain more of the three stilbenes than leaves. The temporal peaks of resveratrol were always ahead of those for oxyresveratrol. The levels of the stilbenes varied in different portions of the varieties of mulberries collected in different season and in the seedlings of Morus atropurpurea Roxb.
语种：
英文
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Sustained Release of Naproxen in a New Kind Delivery System of Carbon Nanotubes Hydrogel
作者：
Peng, Xiahui;Zhuang, Qiang;Peng, Dongming* ;Dong, Qiuli;Tan, Lini;...
期刊：
Iranian Journal of Pharmaceutical Research ,2013年12(4):581-586 ISSN：1735-0328
通讯作者：
Peng, Dongming
作者机构：
[Wang, Xiaomei; Liu, Jingyu; Zhao, Chenxi; Liu, Linqi; Peng, Xiahui] Changsha Univ, Dept Bioengn & Environm Sci, Changsha 410003, Hunan, Peoples R China.;[Peng, Dongming] Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.;[Zhuang, Qiang; Jiao, Feipeng; Tan, Lini; Dong, Qiuli] Cent S Univ, Sch Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.
通讯机构：
[Peng, Dongming] H;Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.
关键词：
Chitosan;Drug delivery;Hydrogel;Multi-walled carbon nanotubes;Release kinetics
摘要：
In this paper, carbon nanotubes (CNTs) were added into chitosan (CS) hydrogels in the form of chitosan modified CNTs (CS-CNTs) composites to prepare carbon nanotubes hydrogels (CNTs-GEL). The products, named CS-MWCNTs, were characterized by scanning electron microscope (SEM) and Fourier transform infrared (FTIR) spectroscopy. Swelling properties and effect of pH on controlled release performance of the two kinds of hydrogels, CNTs-GEL and pure chitosan hydrogels without CNTs (GEL), were investigated respectively. The results showed that CNTs-GEL possess better controlled release performance than GEL. The releasing equilibrium time of CNTs-GEL was longer than that of GEL in both pH = w7.4 and pH=1.2 conditions, although the release ratios of the model drug are similar in the same pH buffer solutions. It is found that release kinetics is better fitted Ritger-Peppas empirical model indicating a fick-diffusion process in pH = 1.2, while in pH = 7.4 it was non-fick diffusion involving surface diffusion and corrosion diffusion processes. © 2013 by School of Pharmacy.
语种：
英文
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对《临床中药学》教材编写的思考与建议
作者：
陈晓阳;李晟;秦莉花;黄宽;扈自然;...
作者机构：
[陈晓阳; 李晟; 秦莉花; 黄宽; 扈自然; 陈广; 叶丽枝] 湖南中医药大学药学院;[陈晓阳; 李晟; 秦莉花; 黄宽; 扈自然; 陈广; 叶丽枝] 湖南省教育厅中药现代化研究重点实验室;[陈晓阳; 李晟; 秦莉花; 黄宽; 扈自然; 陈广; 叶丽枝] 国家中药管理局药性与药效研究三级实验室;[陈晓阳; 李晟; 秦莉花; 黄宽; 扈自然; 陈广; 叶丽枝] 湖南中医药大学护理学院;[陈晓阳; 李晟; 秦莉花; 黄宽; 扈自然; 陈广; 叶丽枝] 湖南中医药大学中医学院
会议名称：
‘2013第六次临床中药学学术年会暨临床中药学学科建设经验交流会
会议时间：
2013-08-01
会议地点：
郑州
会议论文集名称：
‘2013第六次临床中药学学术年会暨临床中药学学科建设经验交流会论文集
关键词：
高等教育;课程建设;临床中药学
摘要：
基于中医药人才培养目标,从理论概念、药物功效,毒性、参考资料等方面,进行审视与思考,提出要进一步补充完善的建议。
语种：
中文
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正交试验法优选乳核分散片提取工艺研究
作者：
欧阳荣;万艳群;廖建萍;罗懿;谢瑜
期刊：
中国医药指南 ,2013年(2):399-401 ISSN：1671-8194
作者机构：
湖南中医药大学第一附属医院,湖南长沙410073;湖南中医药大学药学院,湖南长沙410073
关键词：
正交试验;分散片;醇提工艺
摘要：
目的优选乳核分散片的最佳提取工艺.方法以有效成分丹参酮ⅡA含量和浸膏得率为考察指标,采用正交试验设计,对乳核分散片的醇提工艺进行优化.结果最佳醇提工艺条件为:加入6倍、4倍量70%乙醇提取两次,分别提取1.0h、0.5h.结论优选的提取工艺合理、快速、简便可行,可为乳核分散片工业化生产提供实验依据.
语种：
中文
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白藜芦醇抗皮肤炎症、癣菌相关机理的研究概述
作者：
秦莉花;陈晓阳;张顺荣;李晟;刘俊杰;...
期刊：
中国中医急症 ,2013年22(11):1922-1923 ISSN：1004-745X
作者机构：
湖南中医药大学护理学院,湖南长沙,410208;湖南中医药大学药学院,湖南长沙410208;湖南省教育厅中药现代化研究重点实验室,湖南长沙410208;国家中药管理局药性与药效研究三级实验室,湖南长沙410208;湖南中医药大学,湖南长沙,410208
关键词：
白藜芦醇;皮肤炎症;癣菌;抗炎;抑菌
摘要：
<正>白藜芦醇（Res）又名芪三芬,属于非黄酮类多酚化合物[1],是植物受到真菌、细菌等病原性微生物进攻或是环境恶化如紫外线照射等不利条件时,产生的植物防御素;作为一种抗毒素,对植物本身起保护作用。在抗生素耐药性广泛出现的今天,从传统中药中进行抗菌药物的发掘和研究,将有助于发挥中药在临床感染中的应用。笔者就Res抗皮肤炎症、癣菌及其相关机理的研究,概述如下。
语种：
中文
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纯化工艺对乙肝宁复方中氨基酸影响
作者：
李仲秋;邹龙;刘辉;李欢欢;夏新华
期刊：
中成药 ,2013年35(5):933-937 ISSN：1001-1528
作者机构：
[李仲秋; 邹龙; 刘辉; 刘辉; 夏新华] 湖南中医药大学药学院
关键词：
乙肝宁;氨基酸;壳聚糖絮凝澄清;醇沉;柱前衍生
摘要：
目的探讨乙肝宁复方氨基酸类吸湿性及壳聚糖絮凝澄清与醇沉工艺纯化后乙肝宁中氨基酸变化规律。方法采用阳离子交换树脂分离乙肝宁水提液中的氨基酸,测定吸湿百分率;将乙肝宁水提液经絮凝澄清与醇沉工艺处理后,用732号阳离子交换树脂富集,采用异硫氰酸苯酯(PITC)柱前衍生,梯度洗脱,测定乙肝宁原浸膏及两种纯化工艺处理后游离氨基酸。结果乙肝宁复方总游离氨基酸吸湿性远远高于乙肝宁复方原浸膏;壳聚糖絮凝澄清法对乙肝宁复方水提液中精氨酸、赖氨酸的保留明显优于醇沉法,而对于脯氨酸的保留,虽不如醇沉法,但仍有较高的保留率。结论壳聚糖絮凝澄清法适合用于乙肝宁复方的纯化研究;壳聚糖絮凝澄清法相对于醇沉法可降低中药浸膏吸湿性的原因与氨基酸无关。
语种：
中文
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二角菱壳和四角菱壳不同提取物抗氧化能力比较研究
作者：
裴刚;胡乔铭;向德标;罗李娜;丁扬洲;...
期刊：
中国药物经济学 ,2013年(S1):225-226 ISSN：1673-5846
作者机构：
湖南中医药大学药学院;[袁园; 丁扬洲; 罗李娜; 向德标; 胡乔铭; 裴刚] 湖南中医药大学
关键词：
氧自由基;二角菱壳;四角菱壳
摘要：
目的：研究不同菱角壳不同溶剂的提取物的抗氧化能力。方法采用DPPH法分别测定二角菱角壳、四角菱角壳氯仿、乙酸乙酯、乙醇、水提取浸膏的抗氧化能力，同时与抗坏血酸、槲皮素、没食子酸标准品进行了对比。结果用同一溶剂提取时，二角菱壳提取浸膏的 IC50值均高于四角菱壳提取浸膏；同一种菱角壳用不同溶剂提取时，其提取浸膏与标准品相比 IC50值为：氯仿浸膏＞水浸膏＞乙醇浸膏＞抗坏血酸＞乙酸乙酯浸膏≈槲皮素＞没食子酸，即其清除 DPPH自由基能力的顺序为：没食子酸＞乙酸乙酯浸膏≈槲皮素＞抗坏血酸＞乙醇浸膏＞水浸膏＞氯仿浸膏。结论菱角壳具有较好的抗氧化能力。四角菱壳抗氧化能力强于二角菱壳，菱角壳乙酸乙酯提取部分清除DPPH自由基的能力最强。
语种：
中文
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