期刊：
Frontiers in Pharmacology,2023年14:1173747 ISSN：1663-9812
通讯作者：
Long, HP
作者机构：
[Zhou, Siqian; Long, HP; Liu, Jian; Long, Hongping] Hunan Univ Chinese Med, Ctr Med Res & Innovat, Hosp 1, Changsha, Peoples R China.;[Ding, Changsong; Zhou, Siqian; Wang, Yajing; Long, HP; Long, Hongping] Hunan Univ Chinese Med, Changsha, Peoples R China.;[Tan, Leihong] Hunan Univ Chinese Med, Dept Pharm, Hosp 2, Changsha, Peoples R China.;[Wang, Yikun] Cent South Univ, Xiangya Hosp 2, Dept Pharm, Changsha, Peoples R China.;[Li, Jing] Cent South Univ, Xiangya Hosp, Dept Pharm, Changsha, Peoples R China.
通讯机构：
[Long, HP ] H;Hunan Univ Chinese Med, Ctr Med Res & Innovat, Hosp 1, Changsha, Peoples R China.;Hunan Univ Chinese Med, Changsha, Peoples R China.
摘要：
Introduction: Corni Fructus (CF) is a Chinese herbal medicine used for medicinal and dietary purposes. It is available commercially in two main forms: raw CF (unprocessed CF) and wine-processed CF. Clinical observations have indicated that wine-processed CF exhibits superior hypoglycemic activity compared to its raw counterpart. However, the mechanisms responsible for this improvement are not well understood. Methods: To address this gap in knowledge, we conducted metabolomics analysis using ultra-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry (UPLC-QTOF-MS) to compare the chemical composition of raw CF and wine-processed CF. Subsequently, network analysis, along with immunofluorescence assays, was employed to elucidate the potential targets and mechanisms underlying the hypoglycemic effects of metabolites in CF. Results: Our results revealed significant compositional differences between raw CF and wine-processed CF, identifying 34 potential markers for distinguishing between the two forms of CF. Notably, wine processing led to a marked decrease in iridoid glycosides and flavonoid glycosides, which are abundant in raw CF. Network analysis predictions provided clues that eight compounds might serve as hypoglycemic metabolites of CF, and glucokinase (GCK) and adenylate cyclase (ADCYs) were speculated as possible key targets responsible for the hypoglycemic effects of CF. Immunofluorescence assays confirmed that oleanolic acid and ursolic acid, two bioactive compounds present in CF, significantly upregulated the expression of GCK and ADCYs in the HepG2 cell model. Discussion: These findings support the notion that CF exerted hypoglycemic activity via multiple components and targets, shedding light on the impact of processing methods on the chemical composition and hypoglycemic activity of Chinese herbal medicine.
作者：
Huang, Ying;Liu, Lianghong;Rong, Chunying*;Lu, Tian;Ayers, Paul W.*;...
期刊：
Journal of Molecular Modeling,2018年24(8):213 ISSN：1610-2940
通讯作者：
Rong, Chunying;Ayers, Paul W.;Liu, Shubin
作者机构：
[Huang, Ying] Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.;[Liu, Lianghong] Hunan Univ Med, Dept Pharm, Huaihua 418000, Hunan, Peoples R China.;[Rong, Chunying] Hunan Normal Univ, Coll Chem & Chem Engn, Minist Educ China, Key Lab Chem Biol & Tradit Chinese Med Res, Changsha 410081, Hunan, Peoples R China.;[Lu, Tian] Beijing Kein Res Ctr Nat Sci, Beijing 100022, Peoples R China.;[Ayers, Paul W.] McMaster Univ, Dept Chem, Hamilton, ON L8S 4M1, Canada.
通讯机构：
[Rong, Chunying] H;[Ayers, Paul W.] M;[Liu, Shubin] U;Hunan Normal Univ, Coll Chem & Chem Engn, Minist Educ China, Key Lab Chem Biol & Tradit Chinese Med Res, Changsha 410081, Hunan, Peoples R China.;McMaster Univ, Dept Chem, Hamilton, ON L8S 4M1, Canada.
会议名称：
International Conference on Systems and Processes in Physics, Chemistry and Biology (ICSPPCB) in honor of Pratim K. Chattaraj on his Sixtieth Birthday
会议时间：
MAR 01-03, 2018
会议地点：
Assam Univ, Silchar, INDIA
会议主办单位：
Assam Univ
关键词：
Strong covalent interaction;Pauli energy;Multiple covalent bond;Density-based descriptor;Quintuple bond
作者机构：
[Ma Lijuan; Long Chunlin] Minzu Univ China, Coll Life & Environm Sci, Beijing 100081, Peoples R China.;[Yang Jun; Wang Zhi; Long Chunlin] Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Peoples R China.;[Wang Zhi] Hunan Univ Chinese Med, Coll Pharm, Changsha 410208, Hunan, Peoples R China.;[Ma Lijuan] Univ Macau, Inst Chinese Med Sci, Taipa, Macau, Peoples R China.
会议名称：
3rd International Conference on Advances in Energy and Environmental Science (ICAEES)
会议时间：
JUL 25-26, 2015
会议地点：
Zhuhai, PEOPLES R CHINA
会议主办单位：
[Long Chunlin;Ma Lijuan] Minzu Univ China, Coll Life & Environm Sci, Beijing 100081, Peoples R China.^[Long Chunlin;Yang Jun;Wang Zhi] Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Peoples R China.^[Wang Zhi] Hunan Univ Chinese Med, Coll Pharm, Changsha 410208, Hunan, Peoples R China.^[Ma Lijuan] Univ Macau, Inst Chinese Med Sci, Taipa, Macau, Peoples R China.
摘要：
It has become an action plan to develop biodiesel in China. The botanical and laboratory investigations together with literature surveys had been used to prospect potential plants for biodiesel development. Based on field surveys and collections of specimens and samples, 422 oil-bearing plant species had been documented from Yunnan, the richest province with plant species diversity in the country. The oil content, acid value, iodine value, saponification value, and fatty acid composition of plant samples had been tested and assessed. The results revealed that Camellia oleifera var. monosperma, Michelia sphaerantha, Euonymus tingens, Daphniphyllum paxianum, Trichosanthes rubriflos, Symplocos chinensis and Daphniphyllum macropodum might be selected as the potential plant species for biodiesel development in China.
作者：
Zhou, Y. D.;Mahdi, F.;Li, K. P.;Milasta, S.;Jekabsons, M. B.;...
作者机构：
[Mahdi, F.; Li, K. P.; Khan, I. A.; Zhou, Y. D.; Nagle, D. G.; Gao, J.] Univ Mississippi, Sch Pharm, Dept Biomol Sci, University, MS 38677 USA.;[Mahdi, F.; Li, K. P.; Khan, I. A.; Zhou, Y. D.; Nagle, D. G.; Gao, J.] Univ Mississippi, Sch Pharm, RIPS, University, MS 38677 USA.;[Li, K. P.] Guangdong Pharmaceut Univ, Dept Pharm, Guangzhou 510006, Guangdong, Peoples R China.;[Green, D. R.; Milasta, S.] St Jude Childrens Res Hosp, Dept Immunol, Memphis, TN 38105 USA.;[Jekabsons, M. B.] Univ Mississippi, Dept Biol, University, MS 38677 USA.
会议名称：
Annual Meeting of the American-Society-of-Pharmacognosy
作者：
Chen, Y.;Li, J.;Li, S. X.;Zhao, J.;Bernier, U. R.;...
作者机构：
[Chen, Y.] Hunan Inst Sci & Tech, Coll Chem & Chem Eng, Yueyang, Peoples R China.;[Chen, Y.; Li, J.; Li, S. X.; Wedge, D. E.] Hunan Univ Chinese Med, Sch Pharm, Changsha, Hunan, Peoples R China.;[Zhao, J.] Nat Ctr Nat Prod Res, Univ, MS USA.;[Becnel, J. J.; Bernier, U. R.] ARS, USDA, Ctr Med Agr & Vet Ent, Gainesville, FL USA.;[Wedge, D. E.; Cantrell, C. L.] ARS, Nat Prod Util Res Unit, USDA, Univ, MS USA.
会议名称：
55th Annual Meeting of the American-Society-of-Pharmacognosy (ASP)
摘要：
Using microcalorimetry, the characteristic metabolic heat flow power-time curves of S. aureus growth affected by Ursodesoxycholic acid and Hyodeoxycholic acid were measured at 37 °C. The thermal-kinetic parameters such as, growth rate constant k, the maximum power output (P
m), the time corresponding to the maximum power output (t
p), total heat-production Q
t
, half inhibitory concentration of the drugs (IC
50) were calculated from the growth curves. For both HDCA and UDCA, with the increasing of concentration, k, P
m, and Q
t
decreased, meanwhile, k–c fit a linear equation, t
p was prolonged correspondingly. Principle component analysis, the results indicated t
p might be the main parameter in evaluating the antibacterial activity of HDCA and UDCA in microcalorimetric method. Combining with t
p and IC
50, the results revealed that the differences and trends of antibacterial activity of these bile acid derivatives were: HDCA > UDCA. Structure-activity relationship (SAR) analysis showed that the α-OH at C-3 and C-6 position at equal pace on the steroid nucleus enhanced the hydrophilicity of HDCA, which led to a stronger antibacterial effect than UDCA. In this study, a useful tool was provided to accurately evaluate the antibacterial effects of bile acid derivatives. The thermolysis curve recorded by microcalorimetry could provide a lot of kinetic and thermodynamic information for the study of growth process of living microbial, which could be helpful in the screening of high efficacy antibacterial agents.
摘要：
This paper focuses on the effect of the venom of the scorpion Buthus martensii on the proliferation of human cervical carcinoma Hela cell line and the related molecular mechanism. MTT test showed that
