期刊：
Materials Science and Engineering C: Materials for Biological Applications,2013年33(7):4416-4426 ISSN：0928-4931
通讯作者：
Wang, Jiali
作者机构：
[Wang, Jue; Tang, Jian; Wang, Jiali; Qin, Ling; Yue, Ye] Chinese Acad Sci, Inst Biomed & Hlth Engn, Ctr Translat Med Res & Dev, Shenzhen 518055, Peoples R China.;[Wang, Jiali; Qin, Ling] Chinese Univ Hong Kong, Dept Orthopaed & Traumatol, Musculoskeletal Res Lab, Hong Kong, Hong Kong, Peoples R China.;[Wang, Kai] Hunan Univ Chinese Med, Sch Humanities & Social Sci, Changsha 410208, Hunan, Peoples R China.;[Li, Weirong; Li, Yangde] Guangdong Innovat Team Biodegradable Magnesium &, Eande 523660, Dongguan, Peoples R China.
通讯机构：
[Wang, Jiali] C;Chinese Univ Hong Kong, Dept Orthopaed & Traumatol, Musculoskeletal Res Lab, Hong Kong, Hong Kong, Peoples R China.
关键词：
Magnesium and its alloys;In vitro;Absorbable;Cytotoxicity;Internal fixation
摘要：
Magnesium alloys have been advocated as potential artificial bone materials due to their biocompatibility and biodegradability. The understanding of their corrosive mechanism in physiological environments is therefore essential for making application-orientated designs. Thus, this in vitro study was designed to assess the effects of CO2 on corrosive behavior of AZ31D to mimic in vivo special ingredient. Electrochemical technologies accompanied with Scanning electron microscope, Fourier transform infrared, X-ray diffraction, Energy dispersive spectroscopy and hydrogen evolution measurement were employed to analyze corrosive rates and mechanisms of AZ31D. Moreover, the biocompatibility of AZ31D was assessed with a direct cell attachment assay and an indirect cytotoxicity test in different diluted extracts. The ion concentrations in extracts were measured using inductively coupled plasma mass spectrometry to offer explanations on the differences of cell viability in the indirect test. The results of the direct cytotoxicity assay showed that the corrosive rate of AZ31D was too rapid to allow for cell adhesion. Extracts diluted less than 20 times would cause adverse effects on cell proliferation, likely due to excessive ions and gas release. Moreover, the presence of CO2 did not cause significant differences on corrosive behavior of AZ31D according to the results of electrochemical testing and hydrogen evolution measurement. This might be caused by the simultaneous process of precipitation and dissolution of MgCO3 due to the penetration role of CO2. This analysis of corrosive atmospheres on the degradation behavior of magnesium alloys would contribute to the design of more scientific in vitro testing systems in the future. (C) 2013 The Authors. Published by Elsevier B.V. All rights reserved.
作者机构：
[徐向阳; 韦昌法] College of Computer and Communication, Hunan Univ., Changsha 410082, China;[韦昌法] College of Humanity and Information Management, Hunan Traditional Chinese Medicine University, Changsha 410208, China
通讯机构：
College of Computer and Communication, Hunan Univ., China
