作者机构：
[丁志高; Zeng X.-Q.; 蔡光先; 林琳; 刘柏炎] Department of Internal Medicine, Human Coll. of Traditional Chin. Med, Changsha 410007 Hunan Province, China
通讯机构：
[Cai, G.-X.] D;Department of Internal Medicine, Human Coll. of Traditional Chin. Med, China
关键词：
脑缺血/中药疗法;补阳还五汤;干细胞;神经系统
摘要：
Aim: To observe the effect of buyang huanwu tang on the reaction of endogenous neural stem cells(NSCs) after focal cerebral ischemia in rats, so as to explore its possible mechanism. Methods: Totally 84 male SD rats were randomly divided into 3 groups: sham operation group, model group and buyang huanwu tang group, focal cerebral ischemic models were induced by the thread occlusion method, which received different treatments, and then the rats were killed at 12 hours, 1, 3, 5 and 7 days after operation. The activation of NSC was determined in all groups with immunohistochemistry. Another 5 rats were taken as the normal control group. Results: A few NSCs were observed mainly at the cerebral cortex in the normal rats, and the number of NSCs was increased after ischemia, especially in the cerebral cortex and the region around ischemia. The activation of NSCs was observed in the hippocampal area of the uninjured side; the number of NSCs began to increase on the 1st day, and reached its peak on the 5th day. The number of NSCs at 1st, 3rd, 5th and 7th days in the model group was 10.3 ± 1.3, 14.2 ± 1.7, 18.3 ± 1.2 and 16.4 ± 1.6, respectively, and that in the buyang huanwu tang group was 10.8 ± 1.1, 15.4 ± 1.4, 23.5 ± 1.1 and 22.0 ± 1.4, respectively, and the differences of NSC number between the two groups at the 5th and 7th days were significant(P < 0.01). Conclusion: NSC is activated and proliferated after focal cerebral ischemia, and buyang huanwu tang can improve the proliferation of NSCs.
摘要：
随着医学模式的转变,生存质量(或称生命质量、生活质量,生命质素等,quality of life,QOL)的概念引入医学研究中,尤其近20年来,健康相关生存质量(health-related quality of life)的研究引人注目,欧美一些发达国家已将其广泛应用于临床试验、卫生政策的制定、卫生资源效益的评价等[1],主要涉及癌症、心脑血管病、老年病及其他慢性病生存质量的测评,人群和患者的健康状况评价,临床治疗方案的评价与选择,预防性干预及保健措施的效果评价,并影响着卫生资源配置与利用的决策等[2,3].
