摘要：
Chronic heart failure (CHF) is a complex clinical syndrome caused by a variety of heart problems, with a high incidence. The 5-year survival rate of patients with clinical symptoms is similar to that of malignant tumors. Wenyang Zhenshuai granules are a safe and effective granule of traditional Chinese medicine components, including aconite, dried ginger, licorice, and red ginseng. In contemporary clinical applications, it is widely used in acute and chronic heart insufficiency, coronary heart disease, and arrhythmia. This research cultured H9C2 cardiomyocytes and divided them into the normal control group, LncRNA-MiR143HG overexpression group, LncRNA-MiR143HG silence group, Adriamycin (ADR) group, ADR + medicated serum group, ADR + LncRNA-MiR143HG overexpression + medicated serogroup, and ADR + LncRNA-MiR143HG silence + medicated serogroup. The cells of each group were treated differently, and the survival rate of each group of cells and the expression levels of LncRNA-MiR143HG/miR-143 and ERK5 were detected at the end of the experiment, and the expression of LncRNA-MiR143HG/miR-143 in H9C2 cardiomyocytes was regulated by Wenyang Zhenshuai granules' impact. The results of this study showed that, in the doxorubicin-induced H9C2 cardiomyocyte injury model, the expression of miR-143 was upregulated, and the expression of LncRNA-MiR143HG and ERK5 was significantly downregulated. Wenyang Zhenshuai granules can downregulate the expression of miR-143 to promote ERK5 protein expression and phosphorylation. The process is regulated by LncRNA-MiR143HG/miR-143, which may be one of its important mechanisms for the treatment of chronic heart failure.
摘要：
Objective To explore the effects and possible mechanism of Wenyang Zhenshuai Granules (温阳振衰颗粒) on cardiac and renal function. Methods A total of 40 SD rats were randomly divided into blank group, model group, Wenyang Zhenshuai group, and enalapril group, with 10 rats in each group. Rat except for the blank group were injected intraperitoneally with 3 mg/kg doxorubicin, once a week for 6 weeks to establish rat model of cardio-renal syndrome. After 3 weeks of modeling, rats in the blank and model groups were given 1 ml/100 g of saline by gavage each time, the Wenyang Zhenshuai group was given Wenyang Zhenshuai Granules by gavage 0.72 g/kg every time, and the enalapril group was given enalapril tablets intragastrically 0.9 mg/kg each time for gavage. All the groups were administrated twice a day for 4 weeks. HE staining was used to observe the pathological changes of renal tissue. The positive rate of apoptosis in myocardium and renal tissue was detected by TUNEL method. Heart function, renal function, serum N-terminal pro-brain natriuretic peptide (NT-pro BNP) and renal injury molecule 1 (KIM-1) content were detected, heart and kidney p38 mitogen-activated protein kinase (p38MAPK), phosphorylated p38 mitogen-activated protein kinase (p-p38MAPK) protein and mRNA expression were detected. Quantitation of plasma exosomes, expression of plasma exosome miR-155 in the heart and kidney were detected. Results Compared with the blank group, damaged heart function and kidney function, increased positive rates of apoptosis in myocardium and kidney tissue, increased serum NT-pro BNP and KIM-1 levels, increased p-p38MAPK protein and p38MAPK mRNA in the heart and kidney was increased, and decreased exosomes miR-155 in the heart, kidney and plasma were found (P<0.05 or P<0.01). Compared with the model group, the above indicators were improved to different degree in the Wenyang Zhenhuai group and the enalapril group (P<0.01). Conclusion Wenyang Zhenshuai Granules can improve the cardiac and renal function of rats with cardio-renal syndrome. The action may be related to increasing exosome miR-155 to inhibit the expression of p-p38MAPK, thus exerting anti-apoptotic effects.
