作者机构：
[常小荣; 邓凯文] Department of Acupuncture and Moxibustion, the First Affiliated Hospital of Hunan University of CM, Changsha 410007, China;[唐闻汉; 周逸群; 贺福元; 杨岩涛; 陶叶琴; 石继连; 刘文龙] College of Pharmaceutics, Hunan University of CM, Changsha 410007, China;[唐闻汉; 周逸群; 贺福元; 杨岩涛; 陶叶琴; 石继连; 刘文龙] Hunan Provincial Key Laboratory of Druggability and Preparation Modification of TCM, Changsha 410007, China;[贺福元] Key laboratory of Property and pharmacodynamic of TCM, State Administration of TCM, Changsha 410007, China;[周逸群; 贺福元; 杨岩涛; 石继连; 刘文龙] Supramolecular Mechanism and Mathematic-Physics Characterization for Chinese Materia Medica, Hunan University of CM, Changsha 410007, China
摘要：
Acupuncture is a traditional Chinese medicine therapy that has been found useful for treating various diseases. The treatments involve the insertion of fine needles at acupoints along specific meridians (meridian specificity). This study aims to investigate the metabolic basis of meridian specificity using proton nuclear magnetic resonance ((1)H NMR)-based metabolomics. Electro-acupuncture (EA) stimulations were performed at acupoints of either Stomach Meridian of Foot-Yangming (SMFY) or Gallbladder Meridian of Foot-Shaoyang (GMFS) in healthy male Sprague Dawley (SD) rats. (1)H-NMR spectra datasets of serum, urine, cortex, and stomach tissue extracts from the rats were analysed by multivariate statistical analysis to investigate metabolic perturbations due to EA treatments at different meridians. EA treatment on either the SMFY or GMFS acupoints induced significant variations in 31 metabolites, e.g., amino acids, organic acids, choline esters and glucose. Moreover, a few meridian-specific metabolic changes were found for EA stimulations on the SMFY or GMFS acupoints. Our study demonstrated significant metabolic differences in response to EA stimulations on acupoints of SMFY and GMFS meridians. These results validate the hypothesis that meridian specificity in acupuncture is detectable in the metabolome and demonstrate the feasibility and effectiveness of a metabolomics approach in understanding the mechanism of acupuncture.
作者机构：
School of Acupuncture, Moxibustion & Tuina, Hunan University of Chinese Medicine, Changsha, 410208;The First Hospital of Hunan University of Chinese Medicine, Changsha, 410007;The Third Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, 310053;[刘灿; 周芝根; 杨茜芸; 常小荣; 刘迈兰] School of Acupuncture, Moxibustion & Tuina, Hunan University of Chinese Medicine, Changsha, 410208;[李波] The First Hospital of Hunan University of Chinese Medicine, Changsha, 410007
通讯机构：
[Mai-lan Liu] S;School of Acupuncture, Moxibustion & Tuina, Hunan University of Chinese Medicine, Changsha, China
关键词：
Acupuncture Therapy;Point, Zusanli (ST 36);Central Mechanism;Functional Magnetic Resonance Imaging;Specificity of Acupoints;Research on Acupoints
摘要：
The contents about the central action mechanisms of needling Zusanli (ST 36) were sorted by summarizing the relevant literatures published in the past 10 years, and it was found that: by comparing acupuncture at Zusanli (ST 36), other acupoints or sham points, most studies showed that Zusanli (ST 36) had relatively specific characteristics in the brain function response; studies on the central action mechanisms of Zusanli (ST 36) were mainly focused on sequelae of cerebral apoplexy, pain, gastrointestinal diseases, weight loss and drug addiction withdrawal and so on; acupuncture manipulations, stimulation methods, individual differences, stimulation quantity, and stimulation duration, etc. could also induce different brain function responses; acupuncture at Zusanli (ST 36) had an after-working effect, also called long-term effect, but with poor repeatability. So, it was suggested that the future studies should focus on the factors that affect the clinical efficacies and experimental results, including the compatibility effects of the acupoints, reproducibility of research results, sample size, acupuncture methods, long-term effects and disease entities.
期刊：
African Journal of Traditional, Complementary and Alternative Medicines,2017年14(2):148-160 ISSN：0189-6016
作者机构：
[Ma, Fu-Qiang; Peng, Li; Yang, Zong-Bao; Wang, Ya-Dong; Wang, Chen-Guang] Medical College, Xiamen University, Xiamen 361102 PRC;[Xie, Yu-Feng] Acupuncture and Moxibustion Department, Shenzhen 2 hospital of Traditional Chinese Medicine (Futian hospital of Traditional Chinese Medicine), Shenzhen 518000 PRC;[Wu, Huan-Gan] Research Institute of Acupuncture and Moxibustion, Shanghai University of Traditional Chinese Medicine, Shanghai 200030 PRC;[Liu, Mi; Chang, Xiao-Rong] Acupuncture and Moxibustion College, Hunan University of Traditional Chinese Medicine, Changsha 330004 PRC;[Yang, Zong-Bao; Chang, Xiao-Rong] Zong-bao Yang: corresponding author, Medical College, Xiamen University, Xiamen 361102, China
摘要：
BACKGROUND: It is well known that gastric mucosa dysplasia and intestinal metaplasia are gastric precancerous lesions (GPL). Moxibustion treatment of Liangmen (ST21) and Zusanli (ST36) alleviated the inflammatory response and dysplasia of gastric mucosa in our previous study. The purpose of this study was to further examine the underlying mechanism of moxibustion treatment of ST21 and ST36 on GPL. MATERIALS AND METHODS: Sixty SD rats were divided into five groups and rats with GPL were treated with either moxibustion (ST), moxibustion (Sham), or vitacoenzyme. B-cell lymphoma 2 (bcl-2), tumor protein p53 (P53) and cellular Myc (C-MYC), which are related to cell apoptosis, proliferating cell nuclear antigen (PCNA), vascular endothelial growth factor (VEGF), argyrophilic nucleolar organizer region proteins (Ag-NORs), which are associated with cell proliferation, and cell signaling proteins, nuclear factor kappa B (NF-kappaB), epidermal growth factor receptor (EGFR) and phosphorylated extracellular signal regulated kinase (p-ERK), were measured after moxibustion treatment. RESULTS: Compared with Control group, gastric mucosa in GPL group showed abnormal mucosal proliferation and pathological mitotic figure, the mRNA expression of bcl-2, P53 and C-MYC increased significantly (P < 0.01), the protein expression of PCNA, VEGF, Ag-NORs and the activity of NF-kappabeta as well as EGFR/ERK signaling proteins also increased significantly (P < 0.01). Moxibustion treatment decreased gastric mucosal proliferation and pathological mitotic figure, down-regulated the mRNA expression of bcl-2, P53, C-MYC (P < 0.01), decreased the protein expression of PCNA, VEGF, Ag-NORs and the activity of NF-kappabeta as well as EGFR/ERK signaling proteins significantly (P < 0.01). But moxibustion treatment of Sham didn't show the same effect on GPL. CONCLUSION: Moxibustion treatment inhibited cell apoptosis and reduced gastric mucosa dysplasia by inhibiting the expression of bcl-2, P53, C-MYC and decreased the activity of NF-kappabeta as well as EGFR/ERK signaling proteins.
作者机构：
[常小荣; 刘迈兰; 周芝根; 刘灿; 杨茜芸] College of Acupuncture-Moxibustion and Massage, Hunan University of CM, Changsha 410208, China;[李波] the First Affiliated Hospital of Hunan University of CM
