作者机构：
[Cheng, Qi-Lai; Liu, Ta-Si] Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.;[Huang, Zhi-Qin; Cheng, Qi-Lai; Li, Hong-Liang] Gannan Med Univ, Sch Pharm, Ganzhou 341000, Jiangxi, Peoples R China.;[Chen, Yi-Jian] Gannan Med Univ, Affiliated Hosp 1, Dept Hematol, Ganzhou 341000, Jiangxi, Peoples R China.
通讯机构：
[Chen, Yi-Jian] G;Gannan Med Univ, Affiliated Hosp 1, Dept Hematol, Ganzhou 341000, Jiangxi, Peoples R China.
关键词：
TUA;Actinidia chinensis Radix;NF-kappa B;I kappa B alpha;NCI-H460 cells
摘要：
A natural ursolic compound, 2 beta, 3 beta, 23-trihydroxy-urs-12-ene-28-olic acid (TUA) was isolated from the root of Actinidia chinensis Planch (A. chinensis Radix). Since a large number of triterpenoid compound has marked anticancer effects toward various types of cancer cell lines in vitro, this study was carried out to investigate the anticancer effect of TUA in non-small cell lung cancer cells (NSCLCCs) and the underlying apoptotic mechanism of TUA was examined in NCI-H460 cell lines. Cell proliferation, apoptosis and cell cycle were measured using a cell counting kit-8 (CCK-8) assay and flow cytometry, respectively. The activity of transcription factor NF-kappa B was determined by EMSA method. The expression of apoptosis- and proliferation-related proteins was determined by western blotting. The effect of TUA on NF-kappa B mRNA expression in NCI-H460 cells was detected by RT-PCR. TUA significantly suppressed the viability of NCI-H460 cells. Also, TUA significantly increased the sub G1 population by cell cycle analysis and in a concentration dependent manner in NCI-H460 cells. Such an effect was accompanied by p65 (NF-kappa B subunit) inactivation by an inhibition of I kappa B alpha phosphorylation, and by inhibition of p65 mRNA expressions. Consistently Overall, our findings suggest that TUA induces apoptosis via inhibition of NF-kappa B (p65) expression level and activation of I kappa B alpha in NCI-H460 cells as a potent anticancer candidate for lung cancer treatment. (C) 2015 Published by Elsevier Ireland Ltd.
作者机构：
[李映辰; 刘塔斯] Hunan University of Chinese Medicine, Changsha, 410208, China;[黄志勤; 李洪亮] School of Pharmacy, Gannan Medical University, Ganzhou, 341000, China;[程齐来] Hunan University of Chinese Medicine, Changsha, 410208, China, School of Pharmacy, Gannan Medical University, Ganzhou, 341000, China
通讯机构：
[Liu, T.-S.] H;Hunan University of Chinese Medicine, Changsha, China
作者：
Peng, J. L.;Peng, Q. X.;Lin, L. M.;Dong, W. W.;Liu, T. S.;...
期刊：
Asian Journal of Chemistry,2014年26(12):3477-3482 ISSN：0970-7077
通讯作者：
Yang, D. J.
作者机构：
[Xia, X. H.; Yang, D. J.; Lin, L. M.; Peng, J. L.; Liu, T. S.] Hunan Univ Tradit Chinese Med, Coll Pharm, Changsha, Hunan, Peoples R China.;[Yang, D. J.; Peng, J. L.; Dong, W. W.] State Key Lab Chinese Med & Mol Pharmacol, Shenzhen, Peoples R China.;[Peng, Q. X.] Hong Kong Baptist Univ, Dept Biol, Hong Kong, Hong Kong, Peoples R China.
通讯机构：
[Yang, D. J.] H;Hunan Univ Tradit Chinese Med, Coll Pharm, Changsha, Hunan, Peoples R China.
关键词：
Ganoderma lucidum;HPLC-DAD;Nucleobase;Nucleoside;Quality control
摘要：
A simple, sensitive and reliable HPLC-DAD method was performed for the simultaneous determination of 13 nucleosides and nucleobases including cytosine, uracil, cytidine, hypoxanthine, uridine, thymine, 2′-deoxyuridine, adenine, inosine, guanosine, thymidine, adenosine and 2′-deoxyadenosine in Ganoderma lucidum and its related species. The analysis was conducted on a phenomenex C18 column (250 mm × 4.6 mm i.d., 5 mm) eluting with a gradient of acetonitrile in water at a flow rate of 0.6 mL/min and the detection wavelength was set as 260 nm. The optimized method attained good linear relation (r2 ≥ 0.9996 for 13 analytes), satisfactory precision (RSD < 1.52 % ) and good recovery (from 98.10 to 101.27 %). The validated method was successfully applied to the simultaneous determination of 13 analytes in 42 samples from Ganoderma lucidum and its related species collected from different areas, which could be beneficial to control their quality.
