作者机构：
[宋岚; 张彩平; 黄春林; 乔新惠] Department of Molecular Biology and Biochemistry, Nanhua University, Hengyang 421001, China;[Xu Z.-J.] Department of Cardiothoracic Surgery, Hunan University of Traditional Chinese Medicine, Changsha 410007, China
通讯机构：
[Song, L.] D;Department of Molecular Biology and Biochemistry, Nanhua University, China
作者机构：
[Song lan] Cent S Univ, Xiangya Sch Med, Dept Pathophysiol, Changsha 410008, Hunan, Peoples R China.;[Xu Zhao-jun] Hunan Univ Tradit Chinese Med, Cardiothorac Surg Affiliated Hosp 1, Changsha 41007, Hunan, Peoples R China.;[Zhang Cai-ping; Tian ying] Nanhua Univ, Coll Life Sci, Dept Biochem & Mol Biol, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Song, L ] ;Cent S Univ, Xiangya Sch Med, Dept Pathophysiol, 110 Xiangya Rd, Changsha 410008, Hunan, Peoples R China.
关键词：
fibroblast;adrenaline;bFGF;TGF-beta 1
摘要：
Adrenaline has been shown to modulate proliferation of mouse fibroblasts, adventitial fibroblasts and synovial B (fibroblasts-like) cells. However, little is known about the response of cultured human hypertrophic scar fibroblasts to adrenaline. In this study, we investigated cell proliferation and involved mechanisms in hypertrophic scar fibroblasts in response to adrenaline. Population doubling time (PDT) assay and MTT assay were performed to determine the cell proliferation and cell viability, respectively. The expression of bFGF and TGF-beta 1 was measured by reverse transcriptase-polymerase chain reaction (RT-PCR) and enzyme linked immunosorbent assay (ELISA). The results showed that adrenaline inhibited proliferation of normal and hypertrophic scar fibroblasts in a dose-dependent manner. Moreover, adrenaline up-regulated the expression of bFGF and down-regulated the expression of TGF-beta 1 in normal and hypertrophic scar fibroblasts. Interestingly incubation with the a receptor antagonist regitine indicated that adrenaline mediated inhibition of cell proliferation and regulation of TGF-beta 1 and bFGF in cultured normal and hypertrophic scar fibroblasts were mediated by the a receptor. These studies suggest that adrenaline inhibits proliferation and alters the expression of TGF-beta 1 and bFGF in human hypertrophic scar fibroblast involving an a receptor mediated pathway.
摘要：
目的:观察沐舒坦在体外循环术后治疗中的应用。方法:1025例心脏直视手术患者根据体外循环术后是否使用沐舒坦将其分为两组:治疗组565例和对照组460例。治疗组术后使用沐舒坦治疗;对照组术后未使用沐舒坦治疗。观察两组术后的早期肺功能,住院死亡率、肺部并发症发生率和平均住院时间情况。结果:治疗组拔除气管导管后3 d PaO2、PaCO2、PaO2/FiO2与对照组比较,差异均有统计学意义(P均<0.05),治疗组肺部感染、肺不张发生率较对照组低(P均<0.05),治疗组平均住院时间短于对照组(P<0.05)。结论:沐舒坦可有效改善体外循环手术后早期肺功能,防治肺部并发症,对缩短住院时间、加速患者康复有一定的作用。
