期刊论文

中文

中国中医药信息杂志

1005-5304

2015

22

3

56-59

10.3969/j.issn.1005-5304.2015.03.015

20110491254:中国博士后科学基金 12JJ3114:湖南省自然科学基金 11C0962:湖南省教育厅项目 2014:湖南省中医临床基础重点学科建设项目

本校为第一机构

目的观察疏肝活血中药对骨髓间充质干细胞(BMSCs)移植心肌缺血再灌注损伤(IRI)大鼠心肌细胞凋亡的影响,探讨其保护心肌的作用机制。方法制备心肌 IRI模型,分离、培养 BMSCs,且移植于IRI大鼠,SD大鼠随机分为假手术组、IRI组、BMSCs组、疏肝活血中药+BMSCs组(联合组)。联合组以疏肝活血中药灌胃,其余各组灌胃等量生理盐水,4周后取心肌组织,采用TUNEL法和免疫组化法检测心肌细胞凋亡及心肌细胞Bcl-2和Bax蛋白表达。结果与 IRI组比较,BMSCs组和联合组心肌细胞凋亡、Bax蛋白表达降低,Bcl-2蛋白表达升高(P

Objective To investigate the effects of soothing liver and activating blood Chinese medicine on myocardial cell apoptosis and related gene expression of BMSCs transplanting on myocardial ischemia reperfusion injury (IRI) of rats;To discuss its mechanism of protecting myocardium. Methods Model of myocardial IRI was established in rats. BMSCs were isolated, cultivated, and transplanted in IRI rats. SD rats were randomly divided into sham-operation group, IRI group, BMSCs group, and combined group. Rats in combined group received gavage with soothing liver and activating blood Chinese medicine, w...