期刊论文

Effect oftPNS on Spinal Cord Injury and the Influence on BDNF Expression＊

中文

现代生物医学进展

1673-6273

2012

12

12

2268-2271,2281

10.13241/j.cnki.pmb.2012.12.010

湖南省教育厅优秀青年项目（09B075）；

本校为第一机构

目的：探讨三七总皂苷（total panax notoginseng saponins,tPNS）对脊髓半横断损伤后对脑源性神经营养因子（Brain-derivedneurotrophic factor,BDNF）表达以及运动功能恢复的作用的影响。方法：大鼠随机分为正常组和实验组,实验组大鼠脊髓T10右侧半横断模型,损伤后15min,腹腔注射三七总皂苷,剂量为20mg.kg^-1,以后每天给药一次,溶媒对照组注射等量生理盐水。术后进行BBB评分和斜板实验检测;动物分别存活1d、3d、7d、14d、28d后,采用免疫荧光化学方法检测脊髓损伤远侧端BDNF表达的变化。结果：BBB评分及斜板实验结果显示,三七总皂苷能明显促进脊髓损伤后运动功能的恢复,尤其是损伤后7d和14d,三...

Objective： To investigate the effect of tPNS on spinal cord injury and the influence on BDNF expression. Methods： The right side of the T 10 spinal cord was transected to establish the spinal cord injury model, tPNS was injected intraperitoneally at the dose of 20mg.kg-1 after spinal cord hemisection injury, once a day. The vehicle control group was administrated with equivalent saline. BBB score and IP score were assessed after spinal cord injury. BDNF expression changes in distal spinal cord were detected by immunohistochemistry at ld, 3d, ...