期刊论文

Effects of the combination of active component extracts from Astragalus membranaceus and Panax notoginseng on apoptosis, reactive oxygen species and mitochondrial membrane potential of PC12 cells with oxidative injury

中文

结合医学学报：英文版

2095-4964

2012

10

10

1127-1134

10.3736/jcim20121009

国家自然科学基金资助项目（No.81102557） 教育部2010年度高等学校博士学科点专项科研基金资助项目（No.20104323i10001） 湖南省高校创新平台开放基金资助项目（No.1IK050） 湖南省教育厅一般项目（No.IIC0963） 湖南省高校科技创新团队支持计划

本校为第一机构

目的:探讨黄芪主要有效成分黄芪甲苷和三七主要有效成分人参皂苷Rg1、人参皂苷Rb1、三七皂苷R1配伍抗氯化钴(cobalt chloride,CoCl2)诱导的PC12细胞氧化损伤的作用及其机制.方法:应用CoCl2诱导经神经生长因子转分化的PC12细胞氧化损伤模型,经不同药物处理后,采用Hocchst 33258荧光染色检测细胞凋亡,罗丹明123荧光染色检测细胞线粒体膜电位(mitochondrial membrane potential,MMP),2 ′,7'-二氯荧光黄双乙酸盐荧光染色检测细胞内活性氧(reactive oxygen species,ROS)水平.结果:CoCl2能引起转分化后的PC12细胞凋亡,同时PC12细胞MMP水平显著降低,ROS生成显著增加.黄芪甲苷、人参皂苷Rg1、人参皂苷R...

OBJECTIVE: To explore the effects and mechanisms of combining astragaloside IV (the effective component of Astragalus membranaceus) with notoginsenoside R1, ginsenoside Rb1 and ginsenoside Rg1 (the effective components of Panax notoginseng) against oxidative injury in PC12 cells induced by cobalt chloride (CoCl(2)). METHODS: CoCl(2) was used to stimulate PC12 cells to induce injury after transdifferentiation with nerve growth factor. Then the PC12 cells were divided into 10 groups and cultured with corresponding drugs. After culture, apoptotic cells were tested by using Hocchst 33258 fluoresce...