期刊论文

Expression level of micro RNA-720 in Breast Cancer

中文

世界核心医学期刊文摘(眼科学分册)

1671-3141

2015

15

71

19-19,16

10.3969/j.issn.1671-3141.2015.71.011

81302328:国家自然科学基金 2013FJ4059:湖南省科技厅科技项目

本校为第一机构

目的检测mi R-720在乳腺癌中的表达水平。方法应用Quantitative stem-loop RT–PCR检测55对石蜡包埋乳腺癌及相应癌旁组织、乳腺癌细胞与永生化乳腺上皮细胞中mi R-720的表达水平。结果 mi R-720在石蜡包埋乳腺癌组织和乳腺癌细胞中均呈低表达。结论 mi R-720在乳腺癌中表达水平降低,可能成为乳腺癌诊断的新的分子标志。

Objective To investigate the expression level of miR-720 in breast cancer(BC).Methods The method of quantitative stem-loop RT–PCR was used to detect the expression levels of miR-720 in 55 pairs of paraffin-embedded BC tissues and corresponding tissues adjacent to carcinoma, BC cells and immortalized mammary gland epithelial cells.Results Expression levels of miR-720 in paraffin-embedded BC tissues and BC cell lines are significantly lower than in the corresponding adjacent tissues and immortalized mammary gland epithelial cells.Conclusion miR-720 is downregulated in...