摘要：
目的检测mi R-720在乳腺癌中的表达水平。方法应用Quantitative stem-loop RT–PCR检测55对石蜡包埋乳腺癌及相应癌旁组织、乳腺癌细胞与永生化乳腺上皮细胞中mi R-720的表达水平。结果 mi R-720在石蜡包埋乳腺癌组织和乳腺癌细胞中均呈低表达。结论 mi R-720在乳腺癌中表达水平降低,可能成为乳腺癌诊断的新的分子标志。
期刊：
DNA and Cell Biology,2013年32(6):302-309 ISSN：1044-5498
通讯作者：
Peng, Dan
作者机构：
[Xu, Min; Shen, Yi; Peng, Dan; Jin, Yi] Cent S Univ, Xiangya Hosp 2, Dept Orthoped, Changsha 410000, Hunan, Peoples R China.;[Liang, Ying] Cent South Univ Forestry & Technol, Natl Engn Lab Rice & Byprod Deep Proc, Changsha, Hunan, Peoples R China.;[Xiao, Bowen] Cent Hosp Changsha, Thorac & Cardiovasc Surg Ward, Changsha, Hunan, Peoples R China.;[Lu, Jing] Hunan Univ Chinese Med, Sch Med, Changsha, Hunan, Peoples R China.
通讯机构：
[Peng, Dan] C;Cent S Univ, Xiangya Hosp 2, Dept Orthoped, Changsha 410000, Hunan, Peoples R China.
摘要：
MicroRNAs are a class of small noncoding RNAs that function as critical gene regulators through targeting mRNAs for translational repression or degradation. In this study, we showed that miR-376c expression level was decreased while transforming growth factor-alpha (TGFA) mRNA expression levels were increased in osteosarcoma tissues and cell lines, and we identified TGFA as a novel direct target of miR-376c. Overexpression of miR-376c suppressed TGFA expression and the expression of its downstream signaling molecule such as epidermal growth factor receptor, and attenuated cell proliferation and invasion. Forced expression of TGFA could partly rescue the inhibitory effect of miR-376c in the cells. Taken together, these findings will shed light on the role and mechanism of miR-376c in regulating osteosarcoma cell growth via miR-376c/TGFA axis, and miR-376c may serve as a potential therapeutic target in osteosarcoma in the future.
