miR-145-5p reduces proliferation and migration of hepatocellular carcinoma by targeting KLF5
作者：
Liang, Hao;Sun, Huiping;Yang, Jinfeng;Yi, Chun
* （ 期刊：
Molecular Medicine Reports ,2018年17(6):8332-8338 ISSN：1791-2997
作者机构：
[Liang, Hao] Cent S Univ, Clin Lab, Xiangya Hosp 2, Changsha 410011, Hunan, Peoples R China.;[Sun, Huiping; Yang, Jinfeng] Cent S Univ, Xiangya Sch Med, Affiliated Canc Hosp, Dept Anesthesiol,Hunan Canc Hosp, Changsha 410013, Hunan, Peoples R China.;[Yi, Chun] Hunan Univ Chinese Med, Dept Pathol, 300 Xueshi Rd, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Yi, Chun] H;Hunan Univ Chinese Med, Dept Pathol, 300 Xueshi Rd, Changsha 410208, Hunan, Peoples R China.
关键词：
Hepatocellular carcinoma;Kruppel-like factor 5;MiR-145-5p;Proliferation
摘要：
MicroRNAs (miRs) are important in hepatocellular carcinoma (HCC) progression. miR-145-5p acts as a tumor suppressor in certain malignancies, however, its role in HCC remains unclear. The present study aimed to perform a functional analysis of miR-145-5p in HCC in order to elucidate its role in the pathogenesis of HCC. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed to analyze tissue and cellular expression of miR-145-5p in HCC. Following miRNA mimics transfection, cell viability, apoptosis and cells migration were determined by Cell Counting kit-8, Annexin V-FITC/propidium iodide staining and Transwell analyses. The target of miR-145-5p was analyzed by luciferase reporter assay and western blot analysis. It was observed that miR-145-5p was significantly decreased in HCC tissues and cell lines. Overexpression of miR-145-5p significantly increased apoptosis, reduced cell proliferation and suppressed HCC cell migration. Kruppel-like factor 5 (KLF5) is regarded as a target of miR-145-5p in HCC cells. In addition, KLF5 overexpression partially attenuated the tumor suppressive effects of miR-145-5p. KLF5 expression was negatively associated with levels of miR-145-5p in HCC tissues. The present study demonstrated that miRNA-145-5p may, by targeting KLF5, partially suppress HCC cell growth and motility. The results of the present study suggested that miRNA-145-5p alteration in HCC may serve a role in the progression of HCC. © 2018 Spandidos Publications. All rights reserved.
语种：
英文
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“互联网+”新形势下医疗教育类APP应用于诊断学教学的探索与分析
期刊：
湖南邮电职业技术学院学报 ,2016年15(03):96-98 ISSN：2095-7661
作者机构：
湖南中医药大学医学院,湖南长沙,410000
关键词：
医疗教育类APP;互联网+;诊断学教学
摘要：
从国家战略层面出发,李克强总理提出\"互联网+\"概念,教育领域应当大力推行各类互联网学习方式。目前手机APP在校园生活中的使用频率越来越高,日益成为高校学生生活的必需品之一。文章以120名临床专业学生为样本,将医疗教育类APP应用于诊断学教学过程中。实验证明在教学过程中,有目的地选择使用手机医疗教育类APP,对学生诊断学学习有明显帮助。
语种：
中文
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MicroRNA-720 suppresses M2 macrophage polarization by targeting GATA3
作者：
Zhong, Yan;Yi, Chun
* （ 期刊：
Bioscience Reports ,2016年36(4):e00363. ISSN：0144-8463
作者机构：
[Zhong, Yan] Cent S Univ, Xiangya Hosp 2, Dept Obstet & Gynaecol, Changsha 410011, Hunan, Peoples R China.;[Yi, Chun] Hunan Univ Chinese Med, Dept Pathol, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Yi, Chun] H;Hunan Univ Chinese Med, Dept Pathol, Changsha 410208, Hunan, Peoples R China.
关键词：
breast cancer;GATA3;macrophage polarization;microRNA-720
摘要：
Macrophages are highly plastic cells with the ability to differentiate into both M1- and M2-polarized phenotypes. As a distinct M2-polarized population, tumour-associated macrophages (TAMs) promote tumorigenesis owing to their pro-angiogenic and immune-suppressive functions in tumour microenvironment. In the present study, we found that the microRNA-720 (miR-720) was down-regulated in TAMs isolated from breast carcinomas and M2-polarization macrophages. Overexpression of miR-720 attenuated M2 phenotype expression and thus inhibited M2 polarization. We further identified GATA binding protein 3 (GATA3), a transcriptional factor that plays an important role in M2 macrophage polarization, was the downstream target of miR-720. Ectopic expression of GATA3 restored the M2 phenotype in miR-720 overexpressed macrophages. Importantly, overexpression of miR-720 inhibited pro-migration behaviour and phagocytic ability of M2-polarized macrophages. Thus, our data suggest that miR-720 plays an important role in regulating M2 macrophage polarization and function. © 2016 The Author(s).
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英文
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miR-720在乳腺癌中的表达水平
作者：
刘佳琴
（ ;李国鸿;逯晶;易纯
（ 期刊：
世界核心医学期刊文摘(眼科学分册) ,2015年15(71):19-19,16 ISSN：1671-3141
作者机构：
湖南中医药大学研究生院,湖南 长沙,410208;湖南中医药大学医学院,湖南 长沙,410208;湖南中医药大学医学院西医诊断学教研室,湖南 长沙,410208;湖南中医药大学医学院病理教研室,湖南 长沙,410208
关键词：
乳腺癌;表达水平
摘要：
目的检测mi R-720在乳腺癌中的表达水平。方法应用Quantitative stem-loop RT–PCR检测55对石蜡包埋乳腺癌及相应癌旁组织、乳腺癌细胞与永生化乳腺上皮细胞中mi R-720的表达水平。结果 mi R-720在石蜡包埋乳腺癌组织和乳腺癌细胞中均呈低表达。结论 mi R-720在乳腺癌中表达水平降低,可能成为乳腺癌诊断的新的分子标志。
语种：
中文
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miR-720 inhibits tumor invasion and migration in breast cancer by targeting TWIST1
作者：
Li, Lin-Zi;Zhang, Chris Zhiyi;Liu, Li-Li;Yi, Chun
（ ;Lu, Shi-Xun;...
期刊：
Carcinogenesis ,2014年35(2):469-478 ISSN：0143-3334
通讯作者：
Yun, Jing-Ping
作者机构：
[Yang, Yuan-Zhong; Zhang, Chris Zhiyi; Lu, Shi-Xun; Yun, Jing-Ping; Liu, Li-Li; Zhang, Zhao-Jie; Peng, Yi-Han; Li, Lin-Zi; Zhou, Xuan] Sun Yat Sen Univ, Ctr Canc, State Key Lab Oncol South China, Collaborat Innovat Ctr Canc Med, Guangzhou 510060, Guangdong, Peoples R China.;[Yang, Yuan-Zhong; Zhang, Chris Zhiyi; Lu, Shi-Xun; Yun, Jing-Ping; Liu, Li-Li; Zhang, Zhao-Jie; Peng, Yi-Han; Li, Lin-Zi; Zhou, Xuan] Sun Yat Sen Univ, Ctr Canc, Dept Pathol, Guangzhou 510060, Guangdong, Peoples R China.;[Yi, Chun] Hunan Univ Chinese Med, Coll Med, Dept Pathol, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Yun, Jing-Ping] S;Sun Yat Sen Univ, Ctr Canc, State Key Lab Oncol South China, Collaborat Innovat Ctr Canc Med, Guangzhou 510060, Guangdong, Peoples R China.
摘要：
Breast cancer is the leading cause of cancer death among females, with tumor metastasis being primarily responsible for breast cancer-associated mortality. Current literatures have shown that microRNAs (miRNAs) are implicated in tumor metastasis. In this study, we found that the expression of miR-720 was significantly downregulated in primary breast cancer, with greater downregulation in metastatic tumors. Statistical analysis of 105 cases of primary human breast cancer demonstrated that decreased expression of miR-720 was correlated with lymph node metastasis. Furthermore, reexpression of miR-720 in breast cancer cells remarkably inhibited cell invasiveness and migration both in vitro and in vivo. Mechanistically, downregulation of TWIST1, a promoter of metastasis that was identified as a direct functional target of miR-720, was attributed to the inhibition of metastasis. Consistent with the reduced TWIST1 levels in breast cancer, reexpression of miR-720 upregulated epithelial markers (E-cadherin and ß-catenin) and downregulated mesenchymal markers (N-cadherin, fibronectin, vimentin and matrix metalloproteinase-2). Expression of miR-720 was inversely associated with TWIST1 in human breast cancer tissues. Knockdown of TWIST1 expression by small interfering RNA exhibited similar effects to reintroduction of miR-720, whereas overexpression of TWIST1 (without the 3'-untranslated region) abrogated miR-720-mediated metastasis inhibition. Collectively, our data indicate that miR-720 is frequently decreased in breast cancer and manifests antimetastatic activity by downregulating TWIST1, presenting a novel mechanism of miRNA-mediated regulation of tumor metastasis. © The Author 2013. Published by Oxford University Press. All rights reserved.
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英文
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