期刊论文

Effect of tPNS on GS expression and motor function recovery after spinal cord injury in rats

中文

湖南中医药大学学报

1674-070X

2012

32

1

23-26+30

10.3969/j.issn.1674-070X.2012.01.006.023.04

湖南省教育厅优秀青年项目（09B075）；

本校为第一机构

目的探讨三七总皂苷（total panax notoginseng saponins,TPNS）对大鼠脊髓半横断损伤后运动功能恢复的作用以及对谷氨酰胺合成酶（glutaminesynthetase,GS）表达的影响。方法将大鼠随机分为正常组、溶媒对照组和TPNS组,实验组大鼠建立脊髓半横断模型,TPNS组损伤处为脊髓右侧T10段。损伤后15 min,腹腔注射剂量为20 mg/kg的三七总皂苷,每天给药1次,溶媒对照组注射等量生理盐水。术后1、3、7、14、21、28 d进行行为学指标检测;采用免疫生物化学方法检测脊髓损伤远侧端GS表达的变化。结果行为学结果表明,该浓度的三七总皂苷能促进大鼠脊髓损伤后运动功能的恢复,其中损伤后7 d和14 d的BBB评分表明,三...

Objective To investigate the effect of total panax notoginseng saponins（tPNS） on motor function recovery after semi-transverse spinal cord injury and its association with expression of glutaminesynthetase（GS）.Methods The rats were randomly divided into normal group,vehicle control group and tPNS group,the rats in experimental group were induced the spinal cord hemisection model,in TPNS group the spinal cord injury was at the right side of the T10th segment.After injury 15 min,dose of 20 mg/kg of PNS were injected into the abdominal cavity,o...