作者机构：
[Liuting Zeng] Department of Rheumatology and Immunology, Nanjing Drum Tower Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Graduate School of Peking Union Medical College, Nanjing, China. zltab2016@hotmail.com;[Kailin Yang] Hunan University of Chinese Medicine, Changsha, China;[Qi He] People's Hospital of Ningxiang City, Ningxiang, China;[Xiaofei Zhu] Fudan University, Shanghai, China;[Zhiyong Long] Department of Rehabilitation Medicine, Guangzhou Panyu Central Hospital, Guangzhou, China
通讯机构：
[Liuting Zeng; Lingyun Sun] D;Department of Rheumatology and Immunology, Nanjing Drum Tower Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Graduate School of Peking Union Medical College, Nanjing, China<&wdkj&>Department of Rheumatology and Immunology, Nanjing Drum Tower Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Graduate School of Peking Union Medical College, Nanjing, China<&wdkj&>Department of Rheumatology and Immunology, The First Affiliated Hospital of Anhui Medical University, Hefei, China
摘要：
Previous randomized controlled trials (RCTs) suggested that gut microbiota-based therapies may be effective in treating autoimmune diseases, but a systematic summary is lacking. Pubmed, EMbase, Sinomed, and other databases were searched for RCTs related to the treatment of autoimmune diseases with probiotics from inception to June 2022. RevMan 5.4 software was used for meta-analysis after 2 investigators independently screened literature, extracted data, and assessed the risk of bias of included studies. A total of 80 RCTs and 14 types of autoimmune disease [celiac sprue, SLE, and lupus nephritis (LN), RA, juvenile idiopathic arthritis (JIA), spondyloarthritis, psoriasis, fibromyalgia syndrome, MS, systemic sclerosis, type 1 diabetes mellitus (T1DM), oral lichen planus (OLP), Crohn’s disease, ulcerative colitis] were included. The results showed that gut microbiota-based therapies may improve the symptoms and/or inflammatory factor of celiac sprue, SLE and LN, JIA, psoriasis, PSS, MS, systemic sclerosis, Crohn’s disease, and ulcerative colitis. However, gut microbiota-based therapies may not improve the symptoms and/or inflammatory factor of spondyloarthritis and RA. Gut microbiota-based therapies may relieve the pain of fibromyalgia syndrome, but the effect on fibromyalgia impact questionnaire score is not significant. Gut microbiota-based therapies may improve HbA1c in T1DM, but its effect on total insulin requirement does not seem to be significant. These RCTs showed that probiotics did not increase the incidence of adverse events. Gut microbiota-based therapies may improve several autoimmune diseases (celiac sprue, SLE and LN, JIA, psoriasis, fibromyalgia syndrome, PSS, MS, T1DM, Crohn’s disease, and ulcerative colitis).
作者机构：
[Hao-Ran Peng; Jie Yu; Zhi-Min Yu; Zhi-Min Jiang; Ting-Ting Yan] Kunming University of Science and Technology, Yunnan 650093, China;[Qiang Zhao] Department of Stomatology, 920 Hospital of Joint Logistics Support Force, PLA, Kunming 650032, China;[Ting Xiao] Changsha Stomatological Hospital, School of Dental Medicine, Hunan University of Chinese Medicine, Changsha, Hunan, China
通讯机构：
[Ting-Ting Yan] K;Kunming University of Science and Technology, Yunnan 650093, China
摘要：
As one of the commonly used methods for healing bone defects, autologous bone transplantation is limited due to the scarcity of bone sources and the side effects. Therefore, bone tissue engineering scaffolds are widely used as bone repair materials. Research has shown that the addition of trace elements can improve the osteogenic performance of hydroxyapatite, such as magnesium and strontium ions that can promote bone mineralization and osteoblast proliferation, and promote the formation of new bone in vitro and in vivo tests. The main research results of this paper are as follows: (1) The pore size of the scaffold is distributed between 10 and 70 μm, and it also has a through hole structure of 300–500 μm; (2) Whisker scaffolders had no obvious cytotoxicity, and the addition of Mg and Sr ions would increase the cell proliferation; (3) The degradation rate of the scaffold is relatively slow; (4) ALP shows that pure hydroxyapatite whisker scaffolds have bone mineralization ability, and magnesium and strontium ions can enhance the bone mineralization ability of hydroxyapatite.
通讯机构：
[Tang, ZL; Qiu, RH ; Chen, Y ] H;Hunan Univ Sci & Technol, Sch Chem & Chem Engn, Key Lab Theoret Organ Chem & Funct Mol, Minist Educ,Hunan Prov Key Lab Controllable Prepar, Xiangtan 411201, Hunan, Peoples R China.;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Adv Catalyt Engn Res Ctr,Minist Educ, Changsha 410082, Peoples R China.;Hunan Univ Chinese Med, Sch Med, Dept Physiol, Changsha 410208, Hunan, Peoples R China.
摘要：
A Sb,N ligand (L-Sb) for Pd-catalyzed double N-arylation of primary amines was developed. This trivalent ligand L-Sb, containing a 5,6,7,12-tetrahydrodibenzo[c,f][1,5]azastibocine skeleton and stable under air and moisture, could be synthesized facilely on a gram scale from chlorostibine (1) and cyclopentylmagnesium bromide. L-Sb showed excellent catalytic performance in Pd-2(dba)(3)-catalyzed double N-arylation of 2,2 '-dibromo-1,1 '-biphenyl (2) with primary amines (3), affording functionalized carbazoles in good yields. This Pd-2(dba)(3)/L-Sb-catalyzed double N-arylation, the first example of the application of trivalent organostibines as a ligand in N-arylation, featured the following advantages: small catalyst loading, wide functional group tolerance, good yields, and ease of gram-scale synthesis.
通讯机构：
[Qiu, RH ; Xiong, BQ ; Chen, Y ] H;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China.;Hunan Univ Chinese Med, Sch Med, Dept Physiol, Changsha 410208, Peoples R China.;Hunan Inst Sci & Technol, Dept Chem & Chem Engn, Yueyang 414006, Peoples R China.
摘要：
In this study, we present a nickel-catalyzed reductive C-(sp(3))-Sb coupling of unactivated alkyl chlorides with chlorostibines. This approach is highly versatile, tolerating various functional groups such as acetal, alkene, nitrile, amine, ester, silyl ether, thioether, and various heterocyclic compounds. Notably, the late-stage modification of bioactive molecules and the satisfactory anticancer activity against cancerous MDA-MB-231 also demonstrate the potential application.
摘要：
BACKGROUND: Hyperglycemia is widespread in the world's population, increasing the risk of many diseases. This study aimed to explore the regulatory effect and mechanism of astragaloside IV (ASIV)-mediated endothelial progenitor cells (EPCs) exosomal LINC01963 in endothelial cells (HUVECs) impaired by high glucose. METHODS: Morphologies of exosomes were observed by light microscope and electron microscope. Immunofluorescence was used to identify EPCs and detect the expressions of caspase-1. LINC01963 was detected by quantitative reverse transcription PCR. NLRP3, ASC, and caspase-3 were detected by Western Blot. Nanoparticle tracking analysis was carried out to analyze the exosome diameter. High-throughput sequencing was applied to screen target lncRNAs. The proliferation of endothelial cells was measured by cell counting kit-8 assay. The apoptosis level of HUVECs was detected by flow cytometry and TdT-mediated dUTP Nick-End labeling. The levels of IL-1β, IL-18, ROS, SOD, MDA, and LDH were measured by enzyme-linked immunosorbent assay. RESULTS: ASIV could promote the secretion of the EPC exosome. LINC01963 was obtained by high-throughput sequencing. It was observed that high glucose could inhibit the proliferation, reduce the level of SOD, the expression of NLRP3, ASC, and caspase-1, increase the levels of IL-1β, IL-18, ROS, MDA, and LDH, and promote apoptosis of HUVECs. Whereas LINC01963 could inhibit the apoptosis of HUVECs, the expression of NLRP3, ASC, and caspase-1 increased, and the levels of IL-1β, IL-18, ROS, MDA, and LDH decreased. CONCLUSION: EPCs exosomal LINC01963 play an inhibitory role in high glucose-induced pyroptosis and oxidative stress of HUVECs. This study provides new ideas and directions for treating hyperglycemia and researching exosomal lncRNAs.
作者机构：
[Feng, Xiaolin; Huo, Yi] School of Nursing, Hunan university of Chinese medicine, No. 300, Bachelor Road, Hanpu Science and Education Park, Yuelu District, Changsha City 410208, Hunan Province, China;[Wang, Xiangmeng] Xiangya hospital central south university, 87 Xiangya Road, Changsha 410008, Hunan Province, China;[Luo, Yaoyue] School of Nursing, Hunan university of Chinese medicine, No. 300, Bachelor Road, Hanpu Science and Education Park, Yuelu District, Changsha City 410208, Hunan Province, China. Electronic address: 943629127@qq.com
通讯机构：
[Luo, Yaoyue] S;School of Nursing, Hunan university of Chinese medicine, No. 300, Bachelor Road, Hanpu Science and Education Park, Yuelu District, Changsha City 410208, Hunan Province, China. Electronic address:
关键词：
Competency-based education;Education, nursing;Professional practice
摘要：
BACKGROUND: Currently, nursing students face a significant lack of preparedness in efficiently addressing complex issues. The use of challenge-based learning, a student-centred situational learning method that utilizes practice scenarios to solve complex problems, can help alleviate the challenges in nurse education. However, there remains to be more extensive exploration on the application of challenge-based learning in nurse practice education. OBJECTIVES: This study aims to explore the learning experiences of nursing students in challenge-based learning to gain a deeper understanding of the ways and mechanisms through which challenge-based learning enhances specific learning abilities. DESIGN/METHODS: This study utilized a hermeneutic phenomenological design, employing semi-structured interviews and thematic analysis. SETTINGS: A university in Hunan, China, hosted the nursing skills competition based on the principles of challenge-based learning, targeting senior students from nursing schools in Hunan province. PARTICIPANTS: A total of 24 fourth-year students from six colleges, including one male and twenty-three female students. RESULTS: Two themes and seven sub-themes emerged from the study. Theme 1 Constructing a knowledge system in complex scenarios with sub-themes: Case-based education centred on comprehensive knowledge promotes knowledge linkage; Reverse reasoning promotes knowledge differentiation; Making mistakes helps finding the correct path of knowledge; Traps inspire identification of potential problems. Theme 2 Inquiry in uncertainty with sub-themes: Unexpected changes promote exploration of general principles; Combining knowledge with diverse case scenarios promotes flexible thinking; The uncertainty of knowledge fosters independent thinking. CONCLUSIONS: The complexity and variability inherent in challenging learning situations promote the construction of students' knowledge systems and the cultivation of an inquisitive spirit. The identification of these mechanisms contributes to the optimization of instructional environment design, the development of a culture of continuous learning and innovation, and effectively tackling complex issues within an ever-evolving global context.
作者机构：
[Xie, Ya-Jia; Tian, Sha; Huang, Min] State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology,Macao Special Administrative Region of China;[Tian, Sha] College of Integrated Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, Hunan Province, China;[Lu, Lin-Lin] International Institute for Translational Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, China;[Liu, Zhong-Qiu] Guangdong Provincial Key Laboratory of Translational Cancer Research of Chinese Medicines, Joint International Research Laboratory of Translational Cancer Research of Chinese Medicines, State Key Laboratory of Traditional Chinese Medicine Syndrome, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China;[Chen, Jun-Hui] Intervention and Cell Therapy Center, Peking University Shenzhen Hospital,Shenzhen, China. Electronic address: Chenjhpush@outlook.com
通讯机构：
[Fan, Xing-Xing] S;[Chen, Jun-Hui] I;Intervention and Cell Therapy Center, Peking University Shenzhen Hospital,Shenzhen, China. Electronic address:;State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology,Macao Special Administrative Region of China. Electronic address:
摘要：
The emergence of immune checkpoint inhibitors (ICIs) has revolutionized the clinical treatment for tumor. However, the low response rate of ICIs remains the major obstacle for curing patients and effective approaches for patients with primary or secondary resistance to ICIs remain lacking. In this study, immune stimulating agent unmethylated CG-enriched (CpG) oligodeoxynucleotide (ODN) was locally injected into the tumor to trigger a robust immune response to eradicate cancer cells, while anti-CD25 antibody was applied to remove immunosuppressive regulatory T cells, which further enhanced the host immune activity to attack tumor systematically. The combination of CpG and anti-CD25 antibody obtained notable regression in mouse melanoma model. Furthermore, rechallenge of tumor cells in the xenograft model has resulted in smaller tumor volume, which demonstrated that the combinational treatment enhanced the activity of memory T cells. Remarkably, this combinational therapy presented significant efficacy on multiple types of tumors as well and was able to prevent relapse of tumor partially. Taken together, our combinational immunotherapy provides a new avenue to enhance the clinical outcomes of patients who are insensitive or resistant to ICIs treatments.
