作者机构：
School of Acupuncture, Moxibustion & Tuina, Hunan University of Chinese Medicine, Changsha, 410208;Key Laboratory of Correlativity Between Meridian Point and Viscera, State Administration of Traditional Chinese Medicine, Key Laboratory of Correlativity Between Meridian Point and Viscera, State Administration of Traditional Chinese Medicine, Changsha, 410007;Key Laboratory of Correlativity Between Meridian Point and Viscera, State Administration of Traditional Chinese Medicine, Changsha, 410007;Key Laboratory of Biological Information Analysis for Acupuncture and Moxibustion, Department of Education of Hunan Province, Key Laboratory of Biological Information Analysis for Acupuncture and Moxibustion, Department of Education of Hunan Province, Changsha, 410007;[舒文娜; 杨青; 钟欢; 刘密; 常小荣] School of Acupuncture, Moxibustion & Tuina, Hunan University of Chinese Medicine, Changsha, 410208
通讯机构：
[Mi Liu] S;School of Acupuncture, Moxibustion & Tuina, Hunan University of Chinese Medicine, Changsha, China
摘要：
Objective: To observe the effects of moxibustion pretreatment on the protein expressions of epidermal growth factor receptor (EGFR), phosphorylation extracellular signal-regulated kinase 1/2 (p-ERK1/2) and activated protein-1 (AP-1), the key factors of extracellular signal-regulated kinase signaling transduction pathway in gastric tissue of rats with stress-induced gastric mucosal damage, and to discuss the mechanisms of moxibustion therapy in promoting the restoration of damaged gastric mucosa. Methods: Thirty Sprague-Dawley (SD) rats were randomly divided into a normal group, a model group, and a moxibustion group using the random digits table, 10 in each group. Except the rats in the normal group, rats in the other two groups were used to make stress-induced gastric mucosal damage model using restraint and cold stress. Before modeling, rats in the moxibustion group were alternately treated with moxibustion at Zusanli (ST 36) and Zhongwan (CV 12), or Pishu (BL 20) and Weishu (BL 21), once a day, for a total of 8 d. Histological changes of gastric mucosa were observed under the light microscopy, the expression of gastric tissue p-ERK1/2 was detected by immunohistochemistry assay, and the protein levels of EGFR and AP-1 were measured by Western blots. Results: Compared with rats in the normal group, gastric mucosal damage was more serious, and protein expressions of gastric tissue EGFR, p-ERK1/2 and AP-1 increased in the model group (P<0.01, P<0.05, P<0.05). Compared with rats in the model group, gastric mucosal damage was milder, and protein expressions of gastric tissue EGFR, p-ERK1/2 and AP-1 increased in the moxibustion group (all P<0.01). Conclusion: Moxibustion at Zusanli (ST 36), Zhongwan (CV 12), Pishu (BL 20) and Weishu (BL 21) could increase EGFR, p-ERK1/2 and AP-1 expression levels in gastric tissue of stress-induced gastric mucosal damage rats, maintain the information transfer function of ERK signaling transduction pathway, and promote restoration of gastric mucosal damage.
