摘要：
Objective To observe the effect of Chinese material mediea passing through the liver channel （归肝经中药） on inhibition of proliferation and apoptosis of HepG2 cells in vitro and preliminarily investigate the trends of its effects on anti-hepatocellular carcinoma. Methods Zedoary rhizome （莪术）, leech （水蛭） and salvia miltiorrhiza （丹参） passing through the liver channel were selected to compare with hedyotic diffusa （白花蛇舌草） passing through the non-liver channel as the control. The HepG2 cells were treated by extractions from the above drugs. The inhibition of proliferation and 50% inhibitory rate （IC50） were assayed by methyl thiazolyl tetrazolium （MTT） method. The cell growth and apoptosis of HepG2 ceils in vitro which were treated by IC50 drug concentration of all extractions were detected by methods of cell count, flow cytometry and acridine orange/ethidium bromide （AO/EB） staining. The differences of effects were contrasted between zedoary rhizome, leech and salvia miltiorrhiza which pass through liver channel and hedyotic diffusa which passes through non-liver channel. Results The IC50 drug concentration equal to the crude drug content of hedyotis diffusa was 11.39 times more than that of salvia miltiorrhiza, 16.56 times more than that of leech and 168.67 times more than that of zedoray rhizome. The results showed that the reduction rates of cell count and apoptosis induced by zedoray rhizome, leech and salvia extraction which pass through the liver channel were higher than those of hedyotis diffusa extraction which passes through the non-liver channel. The results demonstrated that cell cycle could be prevented from the G0/G1 phase into the S phase by drugs which pass through the liver channel, while the HepG2 cell cycle under treatment of hedyotis diffusa extraction had no significant effect. Conclusion Chinese material medica passing through the liver channel have stronger effect on the inhibition of proliferation and apoptosis of HepG2 cells than th
摘要：
Objective： To observe the effect of Guanxintongluo Decoction（GD, 冠心通络方） on the expression of NF -κB and the mRNA in of AS rabbits damaged with sacculus proprius. Methods： 60 rabbits were bivided randomly into 4 groups, the treatment group, the model group, the sham control group and the normal control group. All the rabbits except those in the normal control group were feeded with hypso - lipoids feedstuff for 4 months. Then the rabbits in the treatment group and the model group were pulled with the sacculus proprius, while those in the sham control group only were phlebotomized. Rabbits in the treatment group were treated with GD, feeding with hypso - lipoids feedstuff. Those in the model group and the sham control group were only provided, with hypso -lipodis feedstuff. After the succeeding of operation in the sacculus proprius, rabbits in the treatment group would be administered ontinuely for 3 days. All the rabbits were killed on the completion of the course of administration defined, and were taken out the abdominal aorta to be fixed, and then to detect the expression of NF -κB and NF -κB mRNA. Results： GD could notedly decrease the expression of NF -κB and NF -κBmRNA in vascular tissues of AS rabbits damaged with sacculus proprius, which is significant compared with the model group. Conclusion： GD can notedly decrease the expression of NF -κB and NF -κBmRNA in the abdominal aorta of AS rabbits damaged with sacculus proprius, and then may prevent the occurance of RS, which may he a part of the mechanism of preventive effect of GD.
